Overview
- Editors:
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Gérard Ailhaud
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Université de Nice-Sophia Antipolis, Nice, France
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Table of contents (26 protocols)
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Front Matter
Pages i-xiii
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- Louis Casteilla, Luc Pénicaud, Béatrice Cousin, Denis Calise
Pages 1-19
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- Saverio Cinti, M. Cristina Zingaretti, Raffaella Cancello, Enzo Ceresi, Plinio Ferrara
Pages 21-51
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- Mario DiGirolamo, Jacqueline B. Fine
Pages 65-75
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- Hans-Georg Joost, Annette Schürmann
Pages 77-82
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- Cecilia Holm, Gunilla Olivecrona, Malin Ottosson
Pages 97-119
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- Raymond Bazin, Pascal Ferré
Pages 121-127
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- Jean-François Tanti, Mireille Cormont, Thierry Grémeaux, Yannick Le Marchand-Brustel
Pages 157-165
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- Eva Degerman, Svante Resjö, Tova Rahn Landström, Vincent Manganiello
Pages 167-180
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- Susan K. Fried, Naima Moustaid-Moussa
Pages 197-212
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- Barbara Cannon, Jan Nedergaard
Pages 213-224
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- Raymond Négrel, Christian Dani
Pages 225-237
About this book
Adipose tissue is recognized to be exquisitely sensitive to hormone action, and is also now recognized as a secretory and endocrine organ required for reproduction and good health. Adipocytes are “smart” cells able within the tissue to communicate with surrounding cells, but also with various organs, particularly via leptin acting on the central nervous system. Brown adipose tissue (BAT) and white adipose tissue (WAT) are known to be distinct tissues, whereas the heterogeneity of WAT depots is well est- lished. Unfortunately, excess WAT leads to obesity, which is the most common health problem in industrialized countries. Therefore, from both a scientific and a technical point of view, the time has come to create a survey of adipose tissues and their neglected adipocytes. In Adipose Tissue Protocols, I have attempted to gather together chapters from all areas of adipose tissue research—from in vivo to in vitro studies—and to provide methods covering a wide variety of techniques, including the choice of adipose tissue depot and of morphological techniques for the study of BAT and WAT; the isolation, subcellular fractionation, and transfection of adipocytes where the low density of these cells must be taken into account; assays of nutrient and ion fluxes and the metabolic aspects of nutrient uptake; assays of lipid-related enzymes; biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT of various species, including human tissue; measurements of adipose secretory products; and assessment of WAT metabolism in vivo.
Editors and Affiliations
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Université de Nice-Sophia Antipolis, Nice, France
Gérard Ailhaud