Overview
- Editors:
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Eric B. Kmiec
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University of Delaware, Newark
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Table of contents (16 protocols)
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- Barbara A. Demeneix, Mohamed Ghorbel, Daniel Goula
Pages 21-35
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- Bruce D. Bethke, Brian Sauer
Pages 75-84
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- Kaarin K. Goncz, Dieter C. Gruenert
Pages 85-99
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- Ayumi Fujita-Kusano, Yasuhiro Naito, Izumu Saito, Ichizo Kobayashi
Pages 101-109
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- Samuel M. Young Jr., Weidong Xiao, Richard Jude Samulski
Pages 111-126
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- Richard J. Bartlett, Jesica M. McCue
Pages 127-156
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- Sun U. Song, Wayne A. Marasco
Pages 157-166
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- Juliette Fivaz, M. Chiara Bassi, Stéphane Pinaud, Larry Richman, Melanie Price, Jovan Mirkovitch
Pages 167-182
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- Karen M. Vasquez, John H. Wilson
Pages 183-200
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- Howard B. Gamper, Irina Afonina, Evgeniy Belousov, Michael W. Reed, Mikhail A. Podyminogin
Pages 201-211
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- Peter B. Kipp, Joyce M. Van Eck, Peter R. Beetham, Gregory D. May
Pages 213-221
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- Halina Sierakowska, Sudhir Agrawal, Ryszard Kole
Pages 223-227
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Back Matter
Pages 235-244
About this book
The potential now exists in many experimental systems to transfer a cloned, modified gene back into the genome of the host organism. In the ideal situation, the cloned gene is returned to its homologous location in the genome and becomes inserted at the target locus. This process is a controlled means for the repair of DNA damage and ensures accurate chromosome disjunction during meiosis. The paradigm for thinking about the mechanism of this p- cess has emerged primarily from two sources: (1) The principles of reaction mechanics have come from detailed biochemical analyses of the RecA protein purified from Escherichia coli; and (2) the principles of information transfer have been derived from genetic studies carried out in bacteriophage and fungi. A compelling picture of the process of homologous pairing and DNA strand exchange has been influential in directing investigators interested in gene t- geting experiments. The ability to find and pair homologous DNA molecules enables ac- rate gene targeting and is the central phenomenon underlying genetic recombi- tion. Biochemically, the overall process can be thought of as a series of steps in a reaction pathway whereby DNA molecules are brought into homologous register, the four-stranded Holliday structure intermediate is formed, hete- duplex DNA is extended, and DNA strands are exchanged. Not much is known about the biochemical pathway leading to homologous recombination in euka- otes.
Reviews
"The book also has several techniques for using oligonucleotides in gene targeting, a methodology that may well revolutionize the entire field." - Cellular and Molecular Biology
Editors and Affiliations
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University of Delaware, Newark
Eric B. Kmiec
