Skip to main content
SpringerLink
Log in
Book cover

DNA'Protein Interactions

Principles and Protocols

  • Book
  • © 2001

Overview

Editors:
  1. Tom Moss

    1. Centre de Recherche en Cancérologie de l’Université Laval, Centre Hopital Universitaire de Québec et Départment de biologie médicale, Université Laval, Québec, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 148)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (43 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. Filter-Binding Assays

    • Peter G. Stockley
    Pages 1-11

  3. Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions

    • Marc-André Laniel, Alain Béliveau, Sylvain L. Guérin
    Pages 13-30

  4. DNase I Footprinting

    • Benoît Leblanc, Tom Moss
    Pages 31-38

  5. Footprinting with Exonuclease III

    • Willi Metzger, Hermann Heumann
    Pages 39-47

  6. Hydroxyl Radical Footprinting

    • Evgeny Zaychikov, Peter Schickor, Ludmilla Denissova, Hermann Heumann
    Pages 49-61

  7. The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA

    • Brenda F. Kahl, Marvin R. Paule
    Pages 63-75

  8. Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper

    • Athanasios G. Papavassiliou
    Pages 77-110

  9. Uranyl Photofootprinting

    • Peter E. Nielsen
    Pages 111-119

  10. Osmium Tetroxide Modification and the Study of DNA-Protein Interactions

    • James A. McClellan
    Pages 121-134

  11. Determination of a Transcription-Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots

    • Athanasios G. Papavassiliou
    Pages 135-149

  12. Diffusible Singlet Oxygen as a Probe of DNA Deformation

    • Malcolm Buckle, Andrew A. Travers
    Pages 151-160

  13. Ultraviolet-Laser Footprinting

    • Johannes Geiselmann, Frederic Boccard
    Pages 161-173

  14. In Vivo DNA Analysis

    • Régen Drouin, Jean-Philippe Therrien, Martin Angers, Stéphane Ouellet
    Pages 175-219

  15. Identification of Protein-DNA Contacts with Dimethyl Sulfate

    • Peter E. Shaw, A. Francis Stewart
    Pages 221-227

  16. Ethylation Interference

    • Ian W. Manfield, Peter G. Stockley
    Pages 229-243

  17. Hydroxyl Radical Interference

    • Peter Schickor, Evgeny Zaychikov, Hermann Heumann
    Pages 245-253

  18. Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting

    • Simon Labbé, Gale Stewart, Olivier LaRochelle, Carl Séguin, Guy G. Poirier
    Pages 255-264

  19. A Competition Assay for DNA Binding Using the Fluorescent Probe ANS

    • Ian A. Taylor, G. Geoff Kneale
    Pages 265-274

  20. Site-Directed Cleavage of DNA by Linker Histone-Fe(II) EDTA Conjugates

    • David R. Chafin, Jeffrey J. Hayes
    Pages 275-290
Back to top

Keywords

About this book

Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.

Reviews

"The book, written by an impressive list of worldwide contributors, will provide readers with nearly all the necessary tools for studying DNA-protein interactions, making it a perfect cell laboratory manual." -Quarterly Review of Biology

"...a great laboratory resource for biochemists and molecular, cellular, and developmental biologists." - Today's Life Science

Editors and Affiliations

  • Centre de Recherche en Cancérologie de l’Université Laval, Centre Hopital Universitaire de Québec et Départment de biologie médicale, Université Laval, Québec, Canada

    Tom Moss

Bibliographic Information

  • Book Title: DNA'Protein Interactions

  • Book Subtitle: Principles and Protocols

  • Editors: Tom Moss

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592082

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • eBook ISBN: 978-1-59259-208-1Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: 656

  • Number of Illustrations: 146 b/w illustrations

  • Topics: Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation