Overview
- Editors:
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Sergey Fedoroff
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Department of Anatomy and Cell Biology, School of Medicine, University of Saskatchewan, Saskatoon, Canada
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Arleen Richardson
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Department of Anatomy and Cell Biology, School of Medicine, University of Saskatchewan, Saskatoon, Canada
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Table of contents (27 protocols)
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Front Matter
Pages i-xxii
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- Valérie Castellani, Jürgen Bolz
Pages 1-12
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- Dominique Muller, Nicolas Toni, Pierre-Alain Buchs, Lorena Parisi, Luc Stoppini
Pages 13-27
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- Carol A. Colton, Meggan Czapiga, Toby N. Behar
Pages 29-37
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- Bernard Rogister, Gustave Moonen
Pages 39-48
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- Robert B. Campenot, Grace Martin
Pages 49-57
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- Mary I. Johnson, Richard P. Bunge, Patrick M. Wood
Pages 95-115
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- Ruth Cole, Jean de Vellis
Pages 117-127
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- V. Wee Yong, Jack P. Antel
Pages 129-138
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- Sergey Fedoroff, Arleen Richardson
Pages 139-147
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- Bernhard H. J. Juurlink, Shawn K. Thorburne, Richard M. Devon
Pages 149-160
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- Angela Gritti, Rossella Galli, Angelo Luigi Vescovi
Pages 173-197
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- Paul Honegger, Florianne Monnet-Tschudi
Pages 199-218
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- Kaoru Murayama, Natalia N. Singh, Angela Helmrich, David W. Barnes
Pages 219-228
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- Sonia N. Yeung, Frank Tufaro
Pages 229-244
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- Paul J. Price, Gregory J. Brewer
Pages 255-264
About this book
The first edition of Protocols for Neural Cell Culture was published in 1992 and the second edition in 1997. Originally, the publication grew outofprotocols used in the Tissue Culture Course given at the University of Saskatchewan. The course was patterned on those given by the Tissue CultureAssociation, first in Toronto, Canada, in 1948, then in Cooperstown, NY, then Denver, CO, and finally in Madison, WI, where the course ended in 1964. The course in Saskatchewan began in 1963 as a month-long international course that included both animal and plant tissue cultures. Over the years the course underwent specialization, first being limited to animal tissue culture, then to an intensive one-week general course. This led to one-week courses especially designed for tissue culture for the study of cancer or of the cardiovascular or the nervous system. In 1989, the Saskatchewan course became part of the Tissue Culture Training Facility of the Neuroscience Network of the Canadian Network of Centres of Excellence. The course and the Training Facility ceased to exist in 1997. The faculty for the Saskatchewan course was drawn from the best labora tories in the world and laboratory protocols from those centers were thoroughly tested in a student laboratory setting for many years.
Reviews
"In addition to covering standard protocols in well-established culture systems, this is one of the first manuals to include a chapter on protocols to culture neural stem cells. Another unique feature is the inclusion of pictures to facilitate the dissection of particular brain areas, which will be of great help for workers not completely familiar with different aspects of developmental neuroanatomy. . .This is a valuable tool for "culturists" of neural cells. This edition updates protocols for the culturing of neural cell types traditionally used in laboratories studying different aspects of neurobiology and it provides easy-to follow instructions for setting up and maintaining cultures of progenitor cells that will be used in many laboratories in years to come."-Doody's Health Science Book review Journal
"...presents a series of excellent neural tissue culture protocols designed for bench use."
-Trends in Cell Biology
"...a valuable resource that will find its place on many laboratory shelves..."
-Trends in Biotechnology
"...the recipes are comprehensive and easy to follow."
-Trends in Neurosciences
"...indispensable for every project in neuroscience."
-Doody's Health Sciences Book Review Journal
