Protocols for Neural Cell Culture

1. Front Matter

   Pages i-xxii

   PDF

2. In Vitro Assays for Axonal Growth and Targeting

   • Valérie Castellani, Jürgen Bolz

   Pages 1-12

3. Interface Organotypic Hippocampal Slice Cultures

   • Dominique Muller, Nicolas Toni, Pierre-Alain Buchs, Lorena Parisi, Luc Stoppini

   Pages 13-27

4. Slice Cultures for Study of Microglia

   • Carol A. Colton, Meggan Czapiga, Toby N. Behar

   Pages 29-37

5. Microexplant Cultures of the Cerebellum

   • Bernard Rogister, Gustave Moonen

   Pages 39-48

6. Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons

   • Robert B. Campenot, Grace Martin

   Pages 49-57

7. Chick Spinal Somatic Motoneurons in Culture

   • Bernhard H. J. Juurlink

   Pages 59-69

8. Primary Cultures of Sympathetic Ganglia

   • Mary I. Johnson

   Pages 71-94

9. Primary Cell Cultures for the Study of Myelination

   • Mary I. Johnson, Richard P. Bunge, Patrick M. Wood

   Pages 95-115

10. Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures

    • Ruth Cole, Jean de Vellis

    Pages 117-127

11. Culture of Glial Cells from Human Brain Biopsies

    • V. Wee Yong, Jack P. Antel

    Pages 129-138

12. Cultures of Astroglia and Microglia from Primary Cultures of Mouse Neopallium

    • Sergey Fedoroff, Arleen Richardson

    Pages 139-147

13. Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium

    • Bernhard H. J. Juurlink, Shawn K. Thorburne, Richard M. Devon

    Pages 149-160

14. Olfactory Ensheathing Cell Cultures

    • Ronald Doucette

    Pages 161-171

15. Cultures of Stem Cells of the Central Nervous System

    • Angela Gritti, Rossella Galli, Angelo Luigi Vescovi

    Pages 173-197

16. Aggregating Neural Cell Cultures

    • Paul Honegger, Florianne Monnet-Tschudi

    Pages 199-218

17. Neural Cell Lines

    • Kaoru Murayama, Natalia N. Singh, Angela Helmrich, David W. Barnes

    Pages 219-228

18. Virus Vectors for Gene Therapy of the Nervous System

    • Sonia N. Yeung, Frank Tufaro

    Pages 229-244

19. Preparation of Substrata for In Vitro Culture of Neurons

    • Paul C. Letourneau

    Pages 245-254

20. Serum-Free Media for Neural Cell Cultures

    • Paul J. Price, Gregory J. Brewer

    Pages 255-264

The first edition of Protocols for Neural Cell Culture was published in 1992 and the second edition in 1997. Originally, the publication grew outofprotocols used in the Tissue Culture Course given at the University of Saskatchewan. The course was patterned on those given by the Tissue CultureAssociation, first in Toronto, Canada, in 1948, then in Cooperstown, NY, then Denver, CO, and finally in Madison, WI, where the course ended in 1964. The course in Saskatchewan began in 1963 as a month-long international course that included both animal and plant tissue cultures. Over the years the course underwent specialization, first being limited to animal tissue culture, then to an intensive one-week general course. This led to one-week courses especially designed for tissue culture for the study of cancer or of the cardiovascular or the nervous system. In 1989, the Saskatchewan course became part of the Tissue Culture Training Facility of the Neuroscience Network of the Canadian Network of Centres of Excellence. The course and the Training Facility ceased to exist in 1997. The faculty for the Saskatchewan course was drawn from the best labora­ tories in the world and laboratory protocols from those centers were thoroughly tested in a student laboratory setting for many years.

     

• brain
• central nervous system
• myelin
• nervous system
• neurons
• neuroscience

"In addition to covering standard protocols in well-established culture systems, this is one of the first manuals to include a chapter on protocols to culture neural stem cells. Another unique feature is the inclusion of pictures to facilitate the dissection of particular brain areas, which will be of great help for workers not completely familiar with different aspects of developmental neuroanatomy. . .This is a valuable tool for "culturists" of neural cells. This edition updates protocols for the culturing of neural cell types traditionally used in laboratories studying different aspects of neurobiology and it provides easy-to follow instructions for setting up and maintaining cultures of progenitor cells that will be used in many laboratories in years to come."-Doody's Health Science Book review Journal

"...presents a series of excellent neural tissue culture protocols designed for bench use."
-Trends in Cell Biology

"...a valuable resource that will find its place on many laboratory shelves..."
-Trends in Biotechnology

"...the recipes are comprehensive and easy to follow."
-Trends in Neurosciences

"...indispensable for every project in neuroscience."
-Doody's Health Sciences Book Review Journal

• Department of Anatomy and Cell Biology, School of Medicine, University of Saskatchewan, Saskatoon, Canada

  Sergey Fedoroff, Arleen Richardson

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