Overview
- Editors:
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Ricardo V. Lloyd
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Mayo Clinic, Rochester, USA
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Table of contents (20 chapters)
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- Long Jin, Xiang Qian, Ricardo V. Lloyd
Pages 27-46
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- Gary W. Procop, Randall Hayden
Pages 47-65
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- George Kontogeorgos, Nikiforos Kapranos, Eleni Thodou
Pages 91-111
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- John L. Frater, Raymond R. Tubbs
Pages 113-128
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- Akira Matsuno, Tadashi Nagashima, R. Yoshiyuki Osamura, Keiichi Watanabe
Pages 129-144
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- Akira Matsuno, Johbu Itoh, Tadashi Nagashima, R. Yoshiyuki Osamura, Keiichi Watanabe
Pages 165-180
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- Yuri E. Nikiforov, Philip N. Howles
Pages 181-207
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- Omar Bagasra, Lisa E. Bobroski, Muhammad Amjad
Pages 209-227
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- Patrick C. Roche, Eric D. Hsi
Pages 229-237
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- Clive R. Taylor, Shan-Rong Shi
Pages 239-265
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- Naoko Sanno, Akira Teramoto, R. Yoshiyuki Osamura
Pages 267-276
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- Mark R. Wick, Lisa A. Cerilli
Pages 323-360
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- Sergio Vidal, Eva Horvath, Kalman Kovacs
Pages 375-402
About this book
The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.
Reviews
"The book nicely covers the hot topics in this area -- laser capture microdissection, in situ hybridization, FISH, tyramide amplification methods, etc. An attractive feature of this book is that the last 10 chapters (of the 20) contain actual "cookbook" protocols (e.g., PCR, confocal laser scanning microscopy, immunohistochemistry, antigen retrieval, etc.) that can be adapted immediately to any laboratory...This book would be useful for those needing a concise reference for how to perform modern molecular tissue-based technology. " -Doody's Health Science Book Review Journal
"This book describes in detail new methods employed in the study of molecular morphology as a basis for the study of human genes and the etiology of diseases. Among the methods are in situ hybridization, in situ detection of infectious agents, the "FISH" technique, PCR, immunocytochemistry, etc. This excellent production has many photos, some in color, and tables and figures. Very useful to those wishing to employ these techniques." - Journal of Pediatric Endocrinology and Metabolism
Editors and Affiliations
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Mayo Clinic, Rochester, USA
Ricardo V. Lloyd
