Skip to main content
SpringerLink
Log in
Book cover
  • Book
  • © 2002

Calcium-Binding Protein Protocols

Volume 2: Methods and Techniques

Editors:

  1. Hans J. Vogel

    1. Department of Biological Sciences, University of Calgary Calgary, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 173)

Sections

Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (29 protocols)

  1. Front Matter

    Pages i-xvii
    PDF

  2. Methods and Techniques to Study

    1. Front Matter

      Pages 1-1
      PDF

    4. Deconvolution of Calcium-Binding Curves

      • Jacques Haiech, Marie-Claude Kilhoffer
      Pages 25-42

    5. Absorption and Circular Dichroism Spectroscopy

      • Stephen R. Martin, Peter M. Bayley
      Pages 43-55

    7. Steady-State Fluorescence Spectroscopy

      • Aalim M. Weljie, Hans J. Vogel
      Pages 75-87

    10. Differential Scanning Calorimetry

      • Maria M. Lopez, George I. Makhatadze
      Pages 113-119

    11. Isothermal Titration Calorimetry

      • Maria M. Lopez, George I. Makhatadze
      Pages 121-126

    12. Multiangle Laser Light Scattering and Sedimentation Equilibrium

      • Leslie D. Hicks, Jean-René Alattia, Mitsuhiko Ikura, Cyril M. Kay
      Pages 127-136

    15. Synthetic Calcium-Binding Peptides

      • Gary S. Shaw
      Pages 175-182

    16. Proteolytic Fragments of Calcium-Binding Proteins

      • Richard D. Brokx, Hans J. Vogel
      Pages 183-193

    19. Calcium-43 NMR of Calcium-Binding Proteins

      • Torbjörn Drakenberg
      Pages 217-230
Back to top

About this book

Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.
Back to top

Editors and Affiliations

  • Department of Biological Sciences, University of Calgary Calgary, Canada

    Hans J. Vogel

Back to top

Bibliographic Information

  • Book Title: Calcium-Binding Protein Protocols

  • Book Subtitle: Volume 2: Methods and Techniques

  • Editors: Hans J. Vogel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592591841

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • Hardcover ISBN: 978-0-89603-689-5Published: 24 January 2002

  • Softcover ISBN: 978-1-61737-136-3Published: 10 November 2010

  • eBook ISBN: 978-1-59259-184-8Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 415

  • Topics: Biochemistry, general

Back to top

Publish with us

Policies and ethics

Back to top
Access via your institution

Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Search

Navigation