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Calcium-Binding Protein Protocols

Volume 1: Reviews and Case Studies

  • Book
  • © 2002

Overview

Editors:
  1. Hans J. Vogel

    1. Department of Biological Sciences, University of Calgary, Calgary, Canada

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Part of the book series: Methods in Molecular Biology (MIMB, volume 172)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xv
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  2. Introduction and Reviews

    1. Front Matter

      Pages 1-1
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    2. Calcium-Binding Proteins

      • Hans J. Vogel, Richard D. Brokx, Hui Ouyang
      Pages 3-20

    3. Calcium

      • Robert J.P. Williams
      Pages 21-49

    4. Crystal Structure of Calpain and Insights into Ca2+-Dependent Activation

      • Zongchao Jia, Christopher M. Hosfield, Peter L. Davies, John S. Elce
      Pages 51-67

    5. The Multifunctional S100 Protein Family

      • Claus W. Heizmann
      Pages 69-80

    6. Ca2+Binding to Proteins Containing γ-Carboxyglutamic Acid Residues

      • Egon Persson
      Pages 81-95

    7. The Caseins of Milk as Calcium-Binding Proteins

      • Harold M. Farrell Jr., Thomas F. Kumosinski, Edyth L. Malin, Eleanor M. Brown
      Pages 97-140

  3. Calcium-Binding Proteins: Case Studies

    1. Front Matter

      Pages 141-141
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    2. Preparation of Recombinant Plant Calmodulin Isoforms

      • Raymond E. Zielinski
      Pages 143-149

    3. Isolation of Recombinant CardiacTroponin C

      • John A. Putkey, Wen Liu
      Pages 151-160

    4. Skeletal Muscle Troponin C

      • Joyce R. Pearlstone, Lawrence B. Smillie
      Pages 161-174

    5. Purification of Recombinant Calbindin D9k

      • Eva Thulin
      Pages 175-184

    6. S100 Proteins

      • Jean Christophe Deloulme, Gaëlh Ouengue Mbele, Jacques Baudier
      Pages 185-198

    7. Cadherins

      • Jean-René Alattia, Kit I Tong, Masatoshi Takeichi, Mitsuhiko Ikura
      Pages 199-210

    8. α-Lactalbumin and (Calcium-Binding) Lysozyme

      • Katsutoshi Nitta
      Pages 211-224

    9. Recombinant Annexin II Tetramer

      • Hyoung-Min Kang, Nolan R. Filipenko, Geetha Kassam, David M Waisman
      Pages 225-234

    10. Purification and Characterization of ALG-2 A Novel Apoptosis-Linked Ca2+-Binding Protein

      • Mingjie Zhang, Kevin W.-H. Lo
      Pages 235-242

    11. Crystallization and Structural Details of Ca2+-Induced Conformational Changes in the EF-Hand Domain VI of Calpain

      • Miroslaw Cygler, Pawel Grochulski, Helen Blanchard
      Pages 243-260

    12. Neurocalcin

      • Senadhi Vijay-Kumar, Vinod D. Kumar
      Pages 261-279
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Keywords

About this book

Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

Editors and Affiliations

  • Department of Biological Sciences, University of Calgary, Calgary, Canada

    Hans J. Vogel

Bibliographic Information

  • Book Title: Calcium-Binding Protein Protocols

  • Book Subtitle: Volume 1: Reviews and Case Studies

  • Editors: Hans J. Vogel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592591833

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • Hardcover ISBN: 978-0-89603-688-8Published: 02 January 2002

  • Softcover ISBN: 978-1-61737-135-6Published: 10 November 2010

  • eBook ISBN: 978-1-59259-183-1Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 340

  • Number of Illustrations: 129 b/w illustrations, 4 illustrations in colour

  • Topics: Biochemistry, general

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