Calcium-Binding Protein Protocols

1. Front Matter

   Pages i-xv

   PDF

2. Introduction and Reviews

   1. Front Matter

      Pages 1-1

      PDF

   2. Calcium-Binding Proteins

      • Hans J. Vogel, Richard D. Brokx, Hui Ouyang

      Pages 3-20

   3. Calcium

      • Robert J.P. Williams

      Pages 21-49

   4. Crystal Structure of Calpain and Insights into Ca2+-Dependent Activation

      • Zongchao Jia, Christopher M. Hosfield, Peter L. Davies, John S. Elce

      Pages 51-67

   5. The Multifunctional S100 Protein Family

      • Claus W. Heizmann

      Pages 69-80

   6. Ca2+Binding to Proteins Containing γ-Carboxyglutamic Acid Residues

      • Egon Persson

      Pages 81-95

   7. The Caseins of Milk as Calcium-Binding Proteins

      • Harold M. Farrell Jr., Thomas F. Kumosinski, Edyth L. Malin, Eleanor M. Brown

      Pages 97-140

3. Calcium-Binding Proteins: Case Studies

   1. Front Matter

      Pages 141-141

      PDF

   2. Preparation of Recombinant Plant Calmodulin Isoforms

      • Raymond E. Zielinski

      Pages 143-149

   3. Isolation of Recombinant CardiacTroponin C

      • John A. Putkey, Wen Liu

      Pages 151-160

   4. Skeletal Muscle Troponin C

      • Joyce R. Pearlstone, Lawrence B. Smillie

      Pages 161-174

   5. Purification of Recombinant Calbindin D9k

      • Eva Thulin

      Pages 175-184

   6. S100 Proteins

      • Jean Christophe Deloulme, Gaëlh Ouengue Mbele, Jacques Baudier

      Pages 185-198

   7. Cadherins

      • Jean-René Alattia, Kit I Tong, Masatoshi Takeichi, Mitsuhiko Ikura

      Pages 199-210

   8. α-Lactalbumin and (Calcium-Binding) Lysozyme

      • Katsutoshi Nitta

      Pages 211-224

   9. Recombinant Annexin II Tetramer

      • Hyoung-Min Kang, Nolan R. Filipenko, Geetha Kassam, David M Waisman

      Pages 225-234

   10. Purification and Characterization of ALG-2 A Novel Apoptosis-Linked Ca2+-Binding Protein

       • Mingjie Zhang, Kevin W.-H. Lo

       Pages 235-242

   11. Crystallization and Structural Details of Ca2+-Induced Conformational Changes in the EF-Hand Domain VI of Calpain

       • Miroslaw Cygler, Pawel Grochulski, Helen Blanchard

       Pages 243-260

   12. Neurocalcin

       • Senadhi Vijay-Kumar, Vinod D. Kumar

       Pages 261-279

Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

• Amino acid
• Calcium
• chemistry
• membrane
• protein
• protein family
• proteins

• Department of Biological Sciences, University of Calgary, Calgary, Canada

  Hans J. Vogel

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