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Transgenesis Techniques

Principles and Protocols

  • Book
  • © 2002

Overview

Editors:
  1. Alan R. Clarke

    1. School of Biosciences, Cardiff University, Cardiff, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 180)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
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  2. Topical Reviews in Transgenesis

    1. Front Matter

      Pages 1-23
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    2. Biomedical and Agricultural Applications of Animal Transgenesis

      • Jim McWhir
      Pages 3-23

  3. Transgenesis in Invertebrate and Lower Vertebrate Species

    1. Front Matter

      Pages 25-36
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    2. Gene Transfer in Drosophila

      • Mark J. O’Connor, William Chia
      Pages 27-36

  4. Transgenesis in the Mouse: Oocyte Injection

    1. Front Matter

      Pages 37-70
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    2. Oocyte Injection in the Mouse

      • Gary A. J. Brown, Timothy J. Corbin
      Pages 39-70

  5. Alternative Routes to the Germline

    1. Front Matter

      Pages 71-89
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    2. Adenoviral Infection

      • Tohru Tsukui, Yutaka Toyoda
      Pages 73-82

    3. Retroviral Infection

      • Richard A. Bowen
      Pages 83-90

  6. Transgenesis in the Mouse: The ES Cell Route

    1. Front Matter

      Pages 91-204
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    2. In Vitro Isolation of Murine Embryonic Stem Cells

      • David Wells
      Pages 93-126

    3. Production of Chimeras Derived from Murine Embryonic Stem Cells

      • David Wells
      Pages 127-149

    4. Gene-Targeting Strategies

      • David W. Melton
      Pages 151-173

    5. Cre/loxP Recombination System and Gene Targeting

      • Ralf Kühn, Raul M. Torres
      Pages 175-204

  7. Cryopreservation of Mouse Lines

    1. Front Matter

      Pages 205-251
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    2. Cryopreservation of Transgenic Mouse Lines

      • Jillian M. Shaw, Naomi Nakagata
      Pages 207-228

    3. Ovarian Tissue Transplantation and Cryopreservation

      • Jillian M. Shaw, Alan O. Trounson
      Pages 229-252

  8. Transgenesis in the Rat

    1. Front Matter

      Pages 253-341
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    2. Transgenesis in the Rat

      • Linda J. Mullins, Gillian Brooker, John J. Mullins
      Pages 255-270
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About this book

The past decade has witnessed a spectacular explosion in both the devel- ment and use of transgenic technologies. Not only have these been used to aid our fundamental understanding of biologic mechanisms, but they have also faci- tated the development of a range of disease models that are now truly beginning to impact upon our approach to human disease. Some of the most exciting model systems relate to neurodegenerative disease and cancer, where the availability of appropriate models is at last allowing radically new therapies to be developed and tested. This latter point is of particular significance given the current concerns of the wider public over both the use of animal models and the merits of using genetically modified organisms. Arguably, advances of the greatest significance have been made using mammalian systems—driven by the advent of embryonic stem-cell–based strategies and, more recently, by cloning through nuclear transfer. For this reason, this new edition of Transgenesis Techniques focuses much more heavily on manipulation of the mammalian genome, both in the general discussions and in the provision of specific protocols.

Reviews

From Reviews of the First Edition
"...a must in any laboratory..." -Cellular and Molecular Biology

"...should be useful to anyone currently involved in using transgenesis as a technique..."
-Journal of Heredity
Second Edition:

"...the book remains an impressive and interesting book that will be a useful reference to molecular and developmental geneticists at all levels and potential tool for teaching graduate students and advanced undergraduates." - Human Genetics

"It will provide a valuable source of information. It will also remind you of the enormous potential that transgenesis has to offer the biologist. Transgenic research will underpin many future applications from evaluating viral vectors for gene therapy and vaccination to bioreactors for the production of proteins, from models of disease to screening tools for biological activity, from genome mapping to gene function. This book details protocols to let you participate in this." -Cloning and Stem Cells

Editors and Affiliations

  • School of Biosciences, Cardiff University, Cardiff, UK

    Alan R. Clarke

Bibliographic Information

  • Book Title: Transgenesis Techniques

  • Book Subtitle: Principles and Protocols

  • Editors: Alan R. Clarke

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592591787

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • eBook ISBN: 978-1-59259-178-7Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 351

  • Topics: Cell Biology

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