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Liposome Methods and Protocols

  • Book
  • © 2002

Overview

Editors:
  1. Subhash C. Basu

    1. Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame

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    You can also search for this editor in PubMed   Google Scholar

  2. Manju Basu

    1. Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 199)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
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  2. Physical Properties

    1. Front Matter

      Pages 1-1
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    2. Preparation, Isolation, and Characterization of Liposomes Containing Natural and Synthetic Lipids

      • Subroto Chatterjee, Dipak K. Banerjee
      Pages 3-16

    3. Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems

      • Massimo Masserini, Paola Palestini, Marina Pitto, Vanna Chigorno, Sandro Sonnino
      Pages 17-27

  3. Liposome Fusion/Modulation

    1. Front Matter

      Pages 29-29
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    2. Peptide-Induced Fusion of Liposomes

      • Eve-Isabelle Pécheur, Dick Hoekstra
      Pages 31-48

    3. Liposomes

      • Sujoy Ghosh, Robert Bell
      Pages 49-60

    4. Lipids in Viral Fusion

      • Anu Puri, Maite Paternostre, Robert Blumenthal
      Pages 61-81

  4. Application of Liposomes

    1. Front Matter

      Pages 83-83
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    2. Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells

      • Shimon Gatt, Tama Dinur, Arie Dagan
      Pages 85-106

    3. Micelles and Liposomes in Metabolic Enzyme and Glycolipid Glycosyltransferase Assays

      • Manju Basu, Subhash Basu
      Pages 107-130

    4. Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis

      • Roger K. Bretthauer, Dennis W. Welsh
      Pages 131-143

    5. Therapeutic Uses of Antioxidant Liposomes

      • William L. Stone, Shyamali Mukherjee, Milton Smith, Salil K. Das
      Pages 145-161

    6. Targeted Gene Delivery by Virosomes*

      • Debi P. Sarkar, Komal Ramani, Sandeep K. Tyagi
      Pages 163-173

    7. Liposomes Containing Ligands

      • Sriram Neelamegham, Khushi L. Matta
      Pages 175-191

    8. Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes

      • P. R. Satish, A. Surolia
      Pages 193-202

    9. Use of Liposomes Containing Carbohydrates for Production of Monoclonal Antibodies

      • Reiji Kannagi
      Pages 203-218

    10. Inhibition of Tumor Metastasis by Liposomes Containing Glyco-Replica Peptides

      • Takao Taki, Naoto Oku
      Pages 219-231

    11. Use of Phospholipid Bilayers and Monolayers in Binding Studies of Vitamin K-Dependent Blood Coagulation Proteins

      • Francis J. Castellino, Eric H. Ellison
      Pages 233-243

  5. Back Matter

    Pages 245-249
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About this book

In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting; liposomes in gene transport across plasma and nuclear membranes; liposomes in enzyme therapy in patients with genetic disorders. However, before these areas can be effectively explored, many basic areas in liposome research require elucidation, including: (a) attachment of liposomes to cell surfaces; (b) permeation of liposomes through the plasma membranes; and (c) stability of liposomes in cell or nuclear matrices. None of these areas have been exhaustively explored and liposome researchers have ample opportunities to contribute to our knowledge. The aim of Liposome Methods and Protocols is to bring together a wide range of detailed laboratory protocols covering different aspects of liposome biology in order to assist researchers in those rapidly advancing medical fields mentioned earlier. With this goal in mind, in each protocol chapter we have detailed the materials to be used, followed by a step-by-step protocol. The Notes section of each protocol is also certain to prove particularly useful, since the authors include troubleshooting tips straight from their benchtops, valuable information that is seldom given in restricted methods sections of standard research journals. For this reason we feel that the book will prove especially useful for all researchers in the liposome field.

Reviews

"...those who deal with liposome in their work, this book with detailed laboratory protocols and many notes will be very useful." -Pharmaceutical Research

"Volume 199 of the series Methods in Molecular Biology™, offers an excellent overview of protocols used for the study of liposomes in biology...the book portrays comprehensive reports of the study of the cellular fate of sphingolipids using fluoresrole lipids play in virus fusion, studies of functions of various membrane-associated proteins, specifically of phospholipid binding proteins in glycoprotein biosynthesis and contributions of therapeutical liposome applications." -Molecular Biotechnology

Editors and Affiliations

  • Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame

    Subhash C. Basu, Manju Basu

Bibliographic Information

  • Book Title: Liposome Methods and Protocols

  • Editors: Subhash C. Basu, Manju Basu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592591752

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • Hardcover ISBN: 978-0-89603-845-5Published: 15 May 2002

  • Softcover ISBN: 978-1-61737-217-9Published: 10 November 2010

  • eBook ISBN: 978-1-59259-175-6Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 252

  • Topics: Pharmacy

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