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Thyroid Hormone Receptors

Methods and Protocols

  • Book
  • © 2002

Overview

Editors:
  1. Aria Baniahmad

    1. Genetic Institute, Justus-Liebig University, Giessen, Germany

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Part of the book series: Methods in Molecular Biology (MIMB, volume 202)

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Table of contents (11 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Introduction to Thyroid Hormone Receptors

    • Aria Baniahmad
    Pages 1-12

  3. Null Mutant Mice for Thyroid Hormone Receptors

    • Karine Gauthier, Denise Aubert, Olivier Chassande, Frederic Flamant, Jacques Samarut
    Pages 13-29

  4. Transgenic Targeting of a Dominant Negative Corepressor to Liver and Analyses by cDNA Microarray

    • Xu Feng, Paul Meltzer, Paul M. Yen
    Pages 31-54

  5. The Role of Thyroid Hormone Receptors in the Heart

    • Wolfgang H. Dillmann, Bernd R. Gloss
    Pages 55-70

  6. Analysis of Thyroid Hormone-Dependent Genes in the Brain by In Situ Hybridization

    • Juan Bernal, Ana Guadaño-Ferraz
    Pages 71-90

  7. The v-erbA Oncogene

    • Olivier Gandrillon
    Pages 91-107

  8. Gene Regulation by Thyroid Hormone Receptors

    • Helmut Dotzlaw, Aria Baniahmad
    Pages 109-128

  9. Molecular Analysis of Human Resistance to Thyroid Hormone Syndrome

    • Sunnie M. Yoh, Martin L. Privalsky
    Pages 129-152

  10. Function of Thyroid Hormone Receptors During Amphibian Development

    • Sashko Damjanovski, Laurent M. Sachs, Yun-Bo Shi
    Pages 153-176

  11. Transcriptional Regulation by Thyroid Hormone Receptor in Chromatin

    • Jiemin Wong
    Pages 177-194

  12. Gene Activation by Thyroid Hormone Receptor In Vitro and Purification of the TRAP Coactivator Complex

    • Joseph D. Fondell
    Pages 195-214

  13. Back Matter

    Pages 215-223
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About this book

A panel of outstanding investigators surveys and explains the major cutting-edge methods used in thryroid receptor (TR) research and explains their practical experimental details. Described in step-by-step detail to ensure robust experimental results, the techniques presented cover a wide variety of key areas, including TR in development and knockout (mouse and Xenopus), transcriptional regulation by TRs in both cell-free systems and in living cells, and TR mutant analysis of patients. Additional methods provide powerful tools for the isolation of TR-regulated protein complexes, for studying the oncogene v-Erba in blood cell differentiation, and for target gene analysis in the brain. Microarray chip methods are also presented for analyzing the organs of transgenic mice to identify target genes in the liver.

Editors and Affiliations

  • Genetic Institute, Justus-Liebig University, Giessen, Germany

    Aria Baniahmad

Bibliographic Information

  • Book Title: Thyroid Hormone Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Aria Baniahmad

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592591744

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2002

  • Hardcover ISBN: 978-0-89603-995-7Published: 04 June 2002

  • Softcover ISBN: 978-1-4899-3879-4Published: 09 August 2013

  • eBook ISBN: 978-1-59259-174-9Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 223

  • Topics: Endocrinology

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