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  • Book
  • © 2002

Oxidative Stress Biomarkers and Antioxidant Protocols

Editors:

  1. Donald Armstrong

    1. University of Florida, Gainesville

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 186)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Techniques for Free Radical Derived Biomarkers

    1. Front Matter

      Pages 1-1
      PDF

    2. Human Xanthine Oxidoreductase Determination by a Competitive ELISA

      • Maria Giulia Battelli, Silvia Musiani
      Pages 3-11

    4. Determination of Products of Lipid Oxidation by Infrared Spectroscopy

      • Douglas Borchman, Santosh Sinha
      Pages 21-28

    10. Detection of Certain Peroxynitrite-Induced DNA Modifications

      • Hiroshi Ohshima, László. Virág, Jose Souza, Vladimir Yermilov, Brigitte Pignatelli, Mitsuharu Masuda et al.
      Pages 77-88

    11. Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS

      • José A. Castro, Gerardo D. Castro
      Pages 89-99

    12. Analysis of Aliphatic Amino Acid Alcohols in Oxidized Proteins

      • Bénédicte Morin, Shanlin Fu, Hongjie Wang, Michael J. Davies, Roger T. Dean
      Pages 101-110

    13. Rapid Determination of Glutamate Using HPLC Technology

      • Aqeela Afzal, Mohammed Afzal, Andrew Jones, Donald Armstrong
      Pages 111-115

    14. A Rapid Method for the Quantification of GSH and GSSG in Biological Samples

      • Mohammed Afzal, Aqeela Afzal, Andrew Jones, Donald Armstrong
      Pages 117-122

    15. Protein Carbonyl Measurement by ELISA

      • I. Hendrikje Buss, Christine C. Winterbourn
      Pages 123-128

    18. Oxidation of Cellular DNA Measured with the Comet Assay

      • Andrew R. Collins, Mária DuÅ¡inská
      Pages 147-159
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About this book

The first protocols book, Free Radical and Antioxidant Protocols (1) was published in late 1998. Sections were divided into three parts, covering selected biochemical techniques for measuring oxidative stress, antioxidant (AOX) activity, and combined applications. In choosing the 40 methods to be included in that book, I realized there were considerably more of equal value than that which we could have presented in a single volume. To produce a comprehensive resource, this book and a third are being compiled to expand coverage of the field. A summary of papers (2) published on this important subject emphasizes the continuing rapid growth in oxidative stress investigations relating to our understanding of biochemical reactions, their relevance to pathophysiological mechanisms, how disease may arise, and how therapeutic intervention may be achieved(3). Although there is some overlap between the categories, the ana- sis shown below illustrates where current studies are concentrated and are almost evenly distributed between free radicals and AOX. Over the last 4 yr, there has been a 55% increase in the number of papers published in the area.
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Reviews

"The book could be used effectively by experienced or novice basic science researchers or clinicians since the protocols are written clearly and concisely. Each of the chapters in the book gives specific instructions on how to prepare specimens for analysis. The chapters are clearly written, concise, and should be easy for anyone to follow. . .This book is intended and written as a guide to use in the laboratory. It would be quite useful for anyone wishing to assess many parameters of oxidative activities in biological specimens."-doody's Health Sciences Book Review Journal

"This is a superb book, a second volume edited by Donald Armstrong on this topic...There are 33 chapters in the 322 pages of this text therefore, each is commendably succinct and to the point. There are, unfortunately, far too many chapters to list all the topics covered, but it is extensive and amazing." - Free Radical Biology & Medicine
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Editors and Affiliations

  • University of Florida, Gainesville

    Donald Armstrong

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Bibliographic Information

  • Book Title: Oxidative Stress Biomarkers and Antioxidant Protocols

  • Editors: Donald Armstrong

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592591736

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • Hardcover ISBN: 978-0-89603-850-9Published: 04 April 2002

  • Softcover ISBN: 978-1-61737-222-3Published: 10 November 2010

  • eBook ISBN: 978-1-59259-173-2Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 322

  • Topics: Biochemistry, general

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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