Overview
- Editors:
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Stephen P. Robinson
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Department of Hematology, University College Hospital London, UK
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Andrew J. Stagg
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Antigen Presentation Research Group, Imperial College School of Medicine, at Northwick Park Hospital, Harrow, UK
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Stella C. Knight
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Professor of Immunopathology, Imperial College School of Medicine, London
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Table of contents (38 protocols)
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Front Matter
Pages i-xvii
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Isolation of Dendritic Cells from Animal Tissues
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- Andrew J. Stagg, Fiona Burke, Suzanne Hill, Stella C. Knight
Pages 9-22
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- Fabienne Anjuère, Carlos ArdavÍn
Pages 23-28
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- Gordon MacPherson, Michelle Wykes, Fang-Ping Huang, Chris Jenkins
Pages 29-41
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- Franz Koch, Eckhart Kämpgen, Gerold Schuler, Nikolaus Romani
Pages 43-62
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- Philip A. Stumbles, Jennifer Thomas, Patrick G. Holt
Pages 73-83
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- Lina Lu, Maureen L. Drakes, Angus W. Thomson
Pages 85-95
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Isolation and Characterization of Human Dendritic Cells
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- Steven Patterson, Aaron Rae, Heather Donaghy
Pages 111-120
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- Paul U. Cameron, Gianna Stent
Pages 121-136
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- Christoph U. Brand, Nikhil Yawalkar, Robert E. Hunger, Lasse R. Braathen
Pages 137-144
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- Cornelia D. Richters, Matthias J. Hoekstra, John S. du Pont, Robert W. Kreis, Eduard W. A. Kamperdijk
Pages 145-153
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- Michel M. van den Heuvel, Carin E. G. Havenith, Robert H. J. Beelen
Pages 163-173
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- Allison R. Pettit, Lois Cavanagh, Ranjeny Thomas
Pages 175-187
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Derivation of Animal Dendritic Cells from Progenitor Cells
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Front Matter
Pages 189-189
About this book
Dendritic Cell Protocols provides chapter and verse for many useful practical approaches to the art of studying dendritic cells. The book gives information on the usual techniques for derivation of human dendritic cells from precursor stem cells, such as monocytes. In addition it provides data on the difficult tasks of isolating dendritic cells directly from different tissues; whether dendritic cells from precursor cells or from tissues of mouse or human are required, this book contributes practical information. The last section of the book is devoted to functional aspects of dendritic cells ranging from inf- mation relevant to cell migration to antigen uptake and T cell stimulation. But what is a dendritic cell? The explosion of studies on this cell type over the last few years is breathtaking. We now have so-called “myeloid” and “plas- cytoid” dendritic cells; are they of separate lineages or are they related? There are claims that dendritic cells can be derived from B cells and from granulocytes, as well as from “monocytes. ” Lymphocytes can be switched on or off by dendritic cells. Dendritic cells can preferentially cause Th1 or Th2 responses or some may stimulate B cells directly. When there is so much scope for exploring these new developments, it is particularly important that the data that are established and the practical basis for this work are clearly available, a task that this book fulfils.
Reviews
". . .the purposes of the book are worthwhile and are well attained. . .The book could be used effectively by experienced or beginning researchers in the field since the protocols are written clearly and concisely. There are 38 brief chapters in this book by over 70 authors, who are among the most experienced and knowledgeable researchers in the field. . .Among the many experimental protocols described in this book are methods for isolating, culturing, and establishing cell lines from many organs from murine and human sources. The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to study these cells involved in the first line of defense of the immune system. The book has a utilitarian look and feel. . .It would be quite useful for anyone doing bench research on murine or human dendritic cells."-Doody's Health Sciences Book Review Journal
"The layout of the protocols is in a step-by-step fashion which makes them relatively easy to follow. Moreover, they contain enough of the fine details that are so often disregarded in published scientific manuscripts, thus ensuring their reproducibility. Hence, Dendritic Cell Protocols will prove to be an extremely useful source of practical information for novice DC researchers." - Immunology News
Editors and Affiliations
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Department of Hematology, University College Hospital London, UK
Stephen P. Robinson
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Antigen Presentation Research Group, Imperial College School of Medicine, at Northwick Park Hospital, Harrow, UK
Andrew J. Stagg
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Professor of Immunopathology, Imperial College School of Medicine, London
Stella C. Knight
