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Interleukin Protocols

  • Book
  • © 2001

Overview

Editors:
  1. Luke A. J. O’Neill

    1. Department of Biochemistry and Biotechnology Institute, Trinity College, Dublin, Ireland

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    You can also search for this editor in PubMed   Google Scholar

  2. Andrew Bowie

    1. Department of Pharmacology, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 60)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. General Methods for Assaying Interleukin Protein and mRNA

    1. Front Matter

      Pages 1-1
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    2. ELISAs and Interleukin Research

      • Catherine Greene
      Pages 3-15

    3. Elispot Technique for Assaying Interleukins

      • Almuth Christine Hauer, Mona Bajaj-Elliott
      Pages 17-28

    4. Measurement of Interleukins by ELISPOT Assay with Particular Application to Dual-Color Analysis (Stardust Assay)

      • Yoshihiro Okamoto, Mikio Nishida
      Pages 29-39

    5. Assaying Interleukins Intracellularly by FACS

      • Liam O’Mahony, Maurice O’Donoghue
      Pages 41-48

    6. Basic RT-PCR for Measurement of Cytokine Expression

      • Mona Bajaj-Elliott, Almuth Christine Hauer
      Pages 49-58

    7. Quantitative PCR for Measurement of Cytokine Expression

      • Ellen M. Nilsen, Finn-Eirik Johansen, Per Brandtzaeg
      Pages 59-76

    8. Single Cell RT-PCR for the Detection of Cytokine mRNA

      • Moshe Ligumsky, Hovav Nechushtan, Ehud Razin
      Pages 77-81

    9. Quantitation of Cytokine mRNA by Flash-Type Bioluminescence

      • Jeffrey K. Actor
      Pages 83-97

    10. Measurement of Interleukin mRNA by Northern Blotting Using a Nonradioactive Detection Method

      • Ginette Tardif, John A. Di Battista, Jean-Pierre Pelletier, Johanne Martel-Pelletier
      Pages 99-109

    11. Quantification of Mouse IL-6 and TNF- α mRNA Using Xplore Assays

      • Scott W. Van Arsdell, Kevin P. Murphy, Csaba Pazmany, Diane Erickson, Mark D. Moody
      Pages 111-124

    12. Measurement of Cytokine and Chemokine mRNA Using Nonisotopic Multiprobe RNase Protection Assay

      • Zheng-Ming Wang, Roman Dziarski
      Pages 125-143

    13. Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatography

      • Terry M. Phillips, Benjamin F. Dickens
      Pages 145-155

    14. Detection of Cytokines by Immunohistochemistry

      • Mahmoud Huleihel
      Pages 157-162

    15. Whole Blood Assays and the Influence of Circadian Rhythmicity on Human Cytokine Measurement

      • Nikolai Petrovsky
      Pages 163-174

  3. Illustration of Methodologies Using Individual Interleukins

    1. Front Matter

      Pages 175-175
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    2. Assaying Interleukin-8

      • Paul N. Moynagh
      Pages 177-183

    3. Measurement of Interleukin-2 by Bioassay

      • Lyanne Weston
      Pages 185-192

    4. Assaying Interleukin-15

      • Amos Douvdevani, Michal Weiler, Eli Lewis
      Pages 193-204
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About this book

Interleukins are a family of proteins that regulate the maturation, diff- entiation, or activation of cells involved in immunity and inflammation, and belong to a broader family termed cytokines. Collectively these proteins are the key orchestrators of host defense and the response to tissue injury. There are currently 23 different interleukins (numbered from IL-1 to IL-23), although the full extent of the interleukin family will only become clear upon analysis of the human genome sequence. Most important, interleukins are central to the pathogenesis of a wide range of diseases that involve an immune com- nent, including such conditions as rheumatoid arthritis, multiple sclerosis, ulcerative colitis, psoriasis, and asthma. Interleukins have also been imp- cated in other conditions, including cancer, migraine, myocardial infarction, and depression. In essence, when cells are activated by interleukins, a program of gene expression is initiated in the target cell that alters the cell’s phenotype, leading to enhanced immune reactivity, inflammation, and/or proliferation. Interleukins are therefore at the core of the cellular basis for many diseases. They are the subject of intense investigation by biomedical researchers and the targeting or use of interleukins in the clinic is proceeding apace. Approaches such as t- geting IL-4 in asthma or IL-1 in joint disease are being pursued, and it is likely that in the next 5–10 years a number of new therapies based on either inhib- ing or administering interleukins will be available.

Reviews

"this volume offers protocols for measurement of various interleukins. Most are of sufficient detail so as to serve as a laboratory guide for investigators. Investigators with a specific interest in analyzing a given interleukin will find this volume helpful. "-Doody's Health Sciences Book Review Journal

"Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics." - Journal of Investigational Allergology and Clinical immunology

"A variety of techniques is represented in 36 chapters which are written in a concise, uniform format b y authors who obviously have a lot of hands-on experience with the methods covered...If you are working in this area, put this book on a shelf in the lab and it will not be just recently started graduate students who will gracefully turn to it. - Chembiochem

"The protocols that are described in the book are user friendly, in the sense that they are not only well detailed and easy to follow but also reliable. The researchers that are naive in the field will find a lot of useful information in the book whereas the experienced ones will be able to use the book as a source of additional information on the methods already in use. A must therefore for the psychoneuroendocrinologist with a working interest in the relationships between the (neuroendocrine) brain and the immune system." -Psychoneuroendocriniology

Editors and Affiliations

  • Department of Biochemistry and Biotechnology Institute, Trinity College, Dublin, Ireland

    Luke A. J. O’Neill

  • Department of Pharmacology, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland

    Andrew Bowie

Bibliographic Information

  • Book Title: Interleukin Protocols

  • Editors: Luke A. J. O’Neill, Andrew Bowie

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592591469

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • Hardcover ISBN: 978-0-89603-738-0Published: 12 July 2001

  • Softcover ISBN: 978-1-61737-162-2Published: 09 November 2010

  • eBook ISBN: 978-1-59259-146-6Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XV, 443

  • Topics: Immunology

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