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  • Book
  • © 2001

Renal Cancer

Methods and Protocols

Editors:

  1. Jack H. Mydlo

    1. Temple University School of Medicine, Philadelphia

    View editor publications

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Part of the book series: Methods in Molecular Medicine (MIMM, volume 53)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xii
    PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      PDF

    2. Renal Cell Carcinoma

      • Paul Russo
      Pages 3-33

    3. Pathology of Kidney Tumors

      • David J. Grignon
      Pages 35-51

  3. In Vitro Experimental Techniques

    1. Front Matter

      Pages 53-53
      PDF

    2. Telomerase Assay in Renal Cancer

      • William W. Zhang, Laurence H. Klotz
      Pages 55-67

    3. Comparative Genomic Hybridization

      • Joseph C. Presti Jr.
      Pages 69-80

    4. Analyzing the FHIT Gene by RT-PCR, Western Blotting, and Immunohistochemistry

      • Raffaele Baffa, Coleen M. Calviello, Teresa Druck, Leonard G. Gomella
      Pages 81-93

    5. Detection of Apoptosis in Renal Cell Carcinoma

      • Kenneth J. Pienta, Jeffrey F. Williams
      Pages 95-100

    8. Matrix Metalloproteinases and Their Inhibitors

      • Alexander Kugler, Paul Thelen, Rolf-Hermann Ringert
      Pages 139-150

    10. Laser-Capture Microdissection

      • Greg L. Griewe, Robert Dean, Wei Zhang, Isabella A. Sesterhenn, Shiv Srivastava, Judd W. Moul
      Pages 165-173

    11. Expression of c-met and WT-1

      • Leslie Kushner, Pui Yan Chiu, Peter Pinto, Gary Hal Weis
      Pages 175-191

    12. Molecular Analysis of the von Hippel-Lindau Disease Gene

      • Allen Chernoff, Viera Kasparcova, W. Marston Linehan, Catherine A. Stolle
      Pages 193-216

    13. Experimental Models and Methods in Antibody Targeting of Renal Cell Carcinoma

      • Robert L. Vessella, D. Scott Wilbur, Kent Buhler, Paul H. Lange
      Pages 217-242

  4. In Vivo Experimental Techniques

    1. Front Matter

      Pages 243-243
      PDF

    2. Orthotopic Model of Renal Cell Carcinoma

      • David M. Nanus, Dov Engelstein
      Pages 245-253

    3. Murine Animal Model

      • Nobuyasu Nishisaka, Philo Morse, Richard F. Jones, Ching Y. Wang, Gabriel P. Haas
      Pages 255-264
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About this book

The purpose of Renal Cancer: Methods and Protocols is to introduce the surgeon, clinician, investigator, and research scientist to the basic methods employed in the diagnosis and treatment of renal cancer. Treatment of localized renal cancer is surgical. Treatment of metastasis with resection, radiotherapy, chemotherapy, and immunotherapy has had limited results. Therefore, new avenues of treatment are necessary. In planning this work, I have attempted to incorporate coverage by specialists from a variety of disciplines, each applying their individual expertise in renal cancer therapy. Though many of the participating authors are urologists, there are also valuable contributions from medical oncologists, laboratory inv- tigators, and pathologists. One of the inherent problems in publishing a book that describes molecular techniques, especially those techniques that are rapidly evolving, is that some of these may become obsolete after a few years. However, many of the procedures detailed here are standard approaches that have already wi- stood the test of time. Reverse transcriptase PCR, or RT-PCR, a technique that produces m- tiple copies of selected sequences of DNA, has a sensitivity of a millionfold amplication of a single cell, and thus constitutes a very precise technique for measuring the presence of tumor cells. By combining this technique with genomic hybridization, cadherin,ß and metalloproteinase expression we may then reveal other important factors in the detection, staging, aggressiveness, and treatment of this disease.
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Reviews

"Virtually all potentially relevant in vitro and in vivo methodologies are addressed in this book. The sections describing the respective techniques are well written and stylistically equivalent...This book will be a useful addition to most institutional libraries. It will also be of some value to experienced investigators who already possess hands-on familiarity with a particular technique, but merely wish to adapt such methodology to renal cell cancer systems." -Doody's Health Sciences Book Review Journal

"...the intros are great! The writing style is surprisingly uniform and very readable. There is plentiful reference to clinical reality and a strong emphasis on novel treatments." -ACP News

"...the book is well written and the experimental protocols that were included are described in great detail." - Clinical Immunology
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Editors and Affiliations

  • Temple University School of Medicine, Philadelphia

    Jack H. Mydlo

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Bibliographic Information

  • Book Title: Renal Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Jack H. Mydlo

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592591442

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • Hardcover ISBN: 978-0-89603-828-8Published: 25 January 2001

  • Softcover ISBN: 978-1-61737-208-7Published: 09 November 2010

  • eBook ISBN: 978-1-59259-144-2Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XII, 403

  • Number of Illustrations: 55 b/w illustrations

  • Topics: Oncology

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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