Overview
- Editors:
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J. Clifford Murray
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University of Nottingham and City Hospital, Nottingham, UK
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Table of contents (18 protocols)
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Special Review Article
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- Hua-Tang Zhang, Roy Bicknell
Pages 3-26
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Angiogenesis Protocols in Vivo
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- Clifford Y. Ko, Vivek Dixit, William W. Shaw, Gary Gitnick
Pages 53-57
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- Anthony C. Allison, Luis-F. Fajardo
Pages 59-75
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- Roger M. Phillips, Michael C. Bibby
Pages 87-93
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- Axel Sckell, Michael Leunig
Pages 95-105
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- David C. West, W. Douglas Thompson, Paula G. Sells, Mike F. Burbridge
Pages 107-129
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Angiogenesis Protocols in Vitro
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Front Matter
Pages 143-143
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- Ana M. Schor, Ian Ellis, Seth L. Schor
Pages 145-162
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- Ana M. Schor, Ian Ellis, Seth L. Schor
Pages 163-183
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- Mike F. Burbridge, David C. West
Pages 185-204
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Associated Techniques
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Front Matter
Pages 211-211
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About this book
In the last few years, we have been deluged with information on ang- genesis. Scientists and the public at large are exposed daily to this “new” science, not just in specialist journals and texts, but in the tabloid press, where popular articles refer to angiogenic therapies as magic bullets and miracle cures for cancer, arthritis, retinopathies, heart disease, and circulatory problems. Is there no ill this approach will not cure? The fact that so much time, effort, and resource have been and continue to be dedicated to this new science is clear testament to its importance. Yet many fundamental aspects of angiogenesis remain poorly und- stood, in particular cues that activate the process. This fact has to some extent been masked behind a surfeit of fine detail; we can’t see the wood for the trees. Most studies of angiogenesis identify single links in a long chain of events. Furthermore, each study is itself hampered by the limitations of the biological end-point chosen. For instance, though endothelial proliferation may well be necessary for angiogenesis, it is not sufficient. Therefore, measuring endothelial proliferation in response to a novel growth factor, and on the basis of this obs- vation, stating that the factor is “angiogenic,” is unsound logic. It is important that researchers in this field, and perhaps more importantly those experimenting at its periphery, recognize the limitations of their chosen biological end-points.
Reviews
"There is an excellent overview of existing inhibitors of angiogenesis, including both natural and man-made and a review of the biological features that define efficacy and specificity of anti-angiogenic therapies. Most existing in vivo angiogenesis assays are combined in one edition. New microvessel markers are listed to allow more accurate microvessel counts. The details on assays that were proprietary only to a few labs nationwide, such as dorsal skinfold, chamber, and corneal neovascularization assays, are now available to the general science community. Given the well known difficulties of transfection of the primary endothelial cultures, the chapter summarizing information available on transfection and transduction of these cells is particularly useful. . .This is the first book offering such a variety of angiogenesis assays to the general reader where the scientific basis is given in accessible language and technical procedures are readily available. Its publication will undoubtedly facilitate new experiments and further therapeutic angiogenesis."-Doody's Health Sciences Book Review Journal
"The book provides an excellent review concerning anti-angiogenesis. Inhibiting angiogenesis is an important topic in cancer control. . .the book would be a convenient reference for any investigator or laboratory interested in angiogenesis. Angiogenesis Protocols is recommended to any research orientated health science library."-E-Streams Electronic Book Review Journal
"Timely and highly practical, Angiogenesis Protocols is an important resource for today's angiogenesis investigator, providing easy access to a wide variety of proven assays suitable for laboratories with greatly differing technical expertise, materials, and financial resources." - Anticancer Research
Editors and Affiliations
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University of Nottingham and City Hospital, Nottingham, UK
J. Clifford Murray
