Skip to main content
SpringerLink
Log in
Book cover
  • Book
  • © 2001

Angiogenesis Protocols

Editors:

  1. J. Clifford Murray

    1. University of Nottingham and City Hospital, Nottingham, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 46)

Sections

Buy it now

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    PDF

  2. Special Review Article

    1. Front Matter

      Pages 1-1
      PDF

    2. Therapeutic Inhibition of Angiogenesis

      • Hua-Tang Zhang, Roy Bicknell
      Pages 3-26

  3. Angiogenesis Protocols in Vivo

    1. Front Matter

      Pages 27-27
      PDF

    3. In Vivo Matrigel Migration and Angiogenesis Assays

      • Katherine M. Malinda
      Pages 47-52

    4. Alginate Microbead Release Assay of Angiogenesis

      • Clifford Y. Ko, Vivek Dixit, William W. Shaw, Gary Gitnick
      Pages 53-57

    5. Disc Angiogenesis Assay

      • Anthony C. Allison, Luis-F. Fajardo
      Pages 59-75

    6. Sponge Implant Model of Angiogenesis

      • Silvia P. Andrade
      Pages 77-86

    7. Hollow Fiber Assay for Tumor Angiogenesis

      • Roger M. Phillips, Michael C. Bibby
      Pages 87-93

    8. Dorsal Skinfold Chamber Preparation in Mice

      • Axel Sckell, Michael Leunig
      Pages 95-105

    9. Angiogenesis Assays Using Chick Chorioallantoic Membrane

      • David C. West, W. Douglas Thompson, Paula G. Sells, Mike F. Burbridge
      Pages 107-129

    10. Corneal Assay for Angiogenesis

      • Marina Ziche
      Pages 131-142

  4. Angiogenesis Protocols in Vitro

    1. Front Matter

      Pages 143-143
      PDF

    2. Collagen Gel Assay for Angiogenesis

      • Ana M. Schor, Ian Ellis, Seth L. Schor
      Pages 145-162

    3. Chemotaxis and Chemokinesis in 3D Macromolecular Matrices

      • Ana M. Schor, Ian Ellis, Seth L. Schor
      Pages 163-183

    4. Rat Aortic Ring

      • Mike F. Burbridge, David C. West
      Pages 185-204

    5. In Vitro Matrigel Angiogenesis Assays

      • M. Lourdes Ponce
      Pages 205-209

  5. Associated Techniques

    1. Front Matter

      Pages 211-211
      PDF
Back to top

About this book

In the last few years, we have been deluged with information on ang- genesis. Scientists and the public at large are exposed daily to this “new” science, not just in specialist journals and texts, but in the tabloid press, where popular articles refer to angiogenic therapies as magic bullets and miracle cures for cancer, arthritis, retinopathies, heart disease, and circulatory problems. Is there no ill this approach will not cure? The fact that so much time, effort, and resource have been and continue to be dedicated to this new science is clear testament to its importance. Yet many fundamental aspects of angiogenesis remain poorly und- stood, in particular cues that activate the process. This fact has to some extent been masked behind a surfeit of fine detail; we can’t see the wood for the trees. Most studies of angiogenesis identify single links in a long chain of events. Furthermore, each study is itself hampered by the limitations of the biological end-point chosen. For instance, though endothelial proliferation may well be necessary for angiogenesis, it is not sufficient. Therefore, measuring endothelial proliferation in response to a novel growth factor, and on the basis of this obs- vation, stating that the factor is “angiogenic,” is unsound logic. It is important that researchers in this field, and perhaps more importantly those experimenting at its periphery, recognize the limitations of their chosen biological end-points.
Back to top

Reviews

"There is an excellent overview of existing inhibitors of angiogenesis, including both natural and man-made and a review of the biological features that define efficacy and specificity of anti-angiogenic therapies. Most existing in vivo angiogenesis assays are combined in one edition. New microvessel markers are listed to allow more accurate microvessel counts. The details on assays that were proprietary only to a few labs nationwide, such as dorsal skinfold, chamber, and corneal neovascularization assays, are now available to the general science community. Given the well known difficulties of transfection of the primary endothelial cultures, the chapter summarizing information available on transfection and transduction of these cells is particularly useful. . .This is the first book offering such a variety of angiogenesis assays to the general reader where the scientific basis is given in accessible language and technical procedures are readily available. Its publication will undoubtedly facilitate new experiments and further therapeutic angiogenesis."-Doody's Health Sciences Book Review Journal

"The book provides an excellent review concerning anti-angiogenesis. Inhibiting angiogenesis is an important topic in cancer control. . .the book would be a convenient reference for any investigator or laboratory interested in angiogenesis. Angiogenesis Protocols is recommended to any research orientated health science library."-E-Streams Electronic Book Review Journal

"Timely and highly practical, Angiogenesis Protocols is an important resource for today's angiogenesis investigator, providing easy access to a wide variety of proven assays suitable for laboratories with greatly differing technical expertise, materials, and financial resources." - Anticancer Research
Back to top

Editors and Affiliations

  • University of Nottingham and City Hospital, Nottingham, UK

    J. Clifford Murray

Back to top

Bibliographic Information

  • Book Title: Angiogenesis Protocols

  • Editors: J. Clifford Murray

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592591434

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2001

  • eBook ISBN: 978-1-59259-143-5Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: X, 267

  • Number of Illustrations: 119 b/w illustrations

  • Topics: Cell Biology

Back to top

Publish with us

Policies and ethics

Back to top
Access via your institution

Buy it now

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Search

Navigation