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Molecular Analysis of Cancer

  • Book
  • © 2002

Overview

Editors:
  1. Jacqueline Boultwood

    1. Leukaemia Research Fund Molecular Haematology Unit, University of Oxford, NDCLS, John Radcliffe Hospital, Oxford, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Carrie Fidler

    1. Leukaemia Research Fund Molecular Haematology Unit, University of Oxford, NDCLS, John Radcliffe Hospital, Oxford, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 68)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Molecular Analysis of Cancer

    • Ken Mills
    Pages 1-5

  3. Detection of Chromosome Abnormalities in Leukemia Using Fluorescence In Situ Hybridization

    • Lyndal Kearney, Sabrina Tosi, Rina J. Jaju
    Pages 7-27

  4. Spectral Karyotyping in Cancer Cytogenetics

    • Eva Hilgenfeld, Cristina Montagna, Hesed Padilla-Nash, Linda Stapleton, Kerstin Heselmeyer-Haddad, Thomas Ried
    Pages 29-44

  5. Comparative Genomic Hybridization Analysis

    • Binaifer R. Balsara, Jianming Pei, Joseph R. Testa
    Pages 45-57

  6. Detection of Chromosomal Deletions by Microsatellite Analysis

    • Rachel E. Ibbotson, Martin M. Corcoran
    Pages 59-65

  7. Detection and Quantification of Leukemia-Specific Rearrangements

    • Andreas Hochhaus
    Pages 67-96

  8. Detection of t(2;5)(p23;q35)Translocation by Long-Range PCR of Genomic DNA

    • Yunfang Jiang, L. Jeffrey Medeiros, Andreas H. Sarris
    Pages 97-105

  9. Use of DNA Fingerprinting to Detect Genetic Rearrangements in Human Cancer

    • Vorapan Sirivatanauksorn, Yongyut Sirivatanauksorn, Arthur B. McKie, Nicholas R. Lemoine
    Pages 107-114

  10. Mutation Analysis of Large Genomic Regions in Tumor DNA Using Single-Strand Conformation Polymorphism

    • Igor Vorechovsky
    Pages 115-124

  11. Mutational Analysis of Oncogenes and Tumor Suppressor Genes in Human Cancer Using Denaturing Gradient Gel Electrophoresis

    • Per Guldberg, Kirsten GrønbÅ“k, Jesper Worm, Per thor Straten, Jesper Zeuthen
    Pages 125-139

  12. Detection of Mutations in Human Cancer Using Nonisotopic RNase Cleavage Assay

    • Marianna Goldrick, James Prescott
    Pages 141-155

  13. Mutational Analysis of the Neurofibromatosis Type 1 Gene in Childhood Myelodysplastic Syndromes Using a Protein Truncation Assay

    • Lucy Side
    Pages 157-170

  14. Mutation Analysis of Cancer Using Automated Sequencing

    • Amanda Strickson, Carrie Fidler
    Pages 171-177

  15. Detection of Differentially Expressed Genes in Cancer Using Differential Display

    • Yineng Fu
    Pages 179-193

  16. Genomewide Gene Expression Analysis Using cDNA Microarrays

    • Chuang Fong Kong, David Bowtell
    Pages 195-204

  17. Gene Expression Profiling in Cancer Using cDNA Microarrays

    • Javed Khan, Lao H. Saal, Michael L. Bittner, Yuan Jiang, Gerald C. Gooden, Arthur A. Glatfelter et al.
    Pages 205-222

  18. Wilms Tumor Gene WT1 as a

    • Haruo Sugiyama
    Pages 223-237

  19. Detection of Aberrant Methylation of the p15 INK4B Gene Promoter

    • Toshiki Uchida
    Pages 239-249

  20. Clonality Studies in Cancer Based on X Chromosome Inactivation Phenomenon

    • John T. Phelan II, Josef T. Prchal
    Pages 251-270
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About this book

Over the past 20 years, technological advances in molecular biology have proven invaluable to the understanding of the pathogenesis of human cancer. The application of molecular technology to the study of cancer has not only led to advances in tumor diagnosis, but has also provided markers for the assessment of prognosis and disease progression. The aim of Molecular Ana- sis of Cancer is to provide a comprehensive collection of the most up-to-date techniques for the detection of molecular changes in human cancer. Leading researchers in the field have contributed chapters detailing practical pro- dures for a wide range of state-of-the-art techniques. Molecular Analysis of Cancer includes chapters describing techniques for the identification of chromosomal abnormalities and comprising: fluor- cent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), and microsatellite analysis. FISH has a pro- nent role in the molecular analysis of cancer and can be used for the detection of numerical and structural chromosomal abnormalities. The recently described SKY, in which all human metaphase chromosomes are visualized in specific colors, allows for the definition of all chromosomal rearrangements and marker chromosomes in a tumor cell. Protocols for the detection of chromosomal re- rangements by PCR and RT-PCR are described, as well as the technique of DNA fingerprinting, a powerful tool for studying somatic genetic alterations in tumorigenesis.

Reviews

"The book covers a broad range of topics and provides many useful suggestions as well as practical guidance for the lab researcher." -Doody's Health Sciences Book Review Journal

"This book gives a good overview of currently available methods for investigating molecular changes in cancers,...it illustrates how you, the cancer researcher, can apply the methods to your oncology problem...The most enjoyable chapters also illustrate the methods with real results, so that you can see what to expect, e.g. chapter 3 on spectral karyotyping...a useful addition in any oncology research laboratory, because it presents this large variety of methods in a compact format, so that someone waiting to try a new approach could easily dip into the book to see what is available." - Molecular Biotechnology

"Comprehensive and state-of-the-art, Molecular Analysis of Cancer not only offers today's researcher a deeper understanding of cancer's molecular pathogenesis, but also provides a detailed examination of each of the major methodologies needed to detect and analyze its associated genetic abnormalities." - Clinical Laboratory

"...good integration between preliminary explanations of techniques and practical instructions with easily available tables or figures." - Proteomics

Editors and Affiliations

  • Leukaemia Research Fund Molecular Haematology Unit, University of Oxford, NDCLS, John Radcliffe Hospital, Oxford, UK

    Jacqueline Boultwood, Carrie Fidler

Bibliographic Information

  • Book Title: Molecular Analysis of Cancer

  • Editors: Jacqueline Boultwood, Carrie Fidler

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592591353

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • Hardcover ISBN: 978-0-89603-622-2Published: 10 October 2001

  • Softcover ISBN: 978-1-61737-102-8Published: 12 December 2011

  • eBook ISBN: 978-1-59259-135-0Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XII, 304

  • Topics: Pathology

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