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  • Book
  • © 2000

Gene Therapy of Cancer

Methods and Protocols

Editors:

  1. Wolfgang Walther

    1. Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Ulrike Stein

    1. Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 35)

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Table of contents (39 protocols)

  1. Front Matter

    Pages i-xvi
    PDF

  2. Experimental Approaces To Cancer Gene Therapy

    1. Front Matter

      Pages 1-436
      PDF

    2. Immunizing Potential of Cytokine-Transduced Tumor Cells

      • Mario P. Colombo, Monica Rodolfo
      Pages 3-26

    3. Particle-Mediated Gene Transfer into Dendritic Cells

      • Thomas Tüting, Andreas Albers
      Pages 27-47

    4. Cancer Gene Therapy With Heat Shock Protein-65 Gene

      • Katalin V. Lukacs, Artit Nakakes
      Pages 49-56

    5. Recombinant Vaccinia Virus MVA for Generation and Analysis of T Cell Responses Against Tumor Associated Antigens

      • Ingo Drexler, Karl Heller, Marion Ohlmann, Volker Erfle, Gerd Sutter
      Pages 57-73

  3. Experimental Approaces To Cancer Gene Therapy

    1. Front Matter

      Pages 1-436
      PDF

    3. Construction of P450-Expressing Tumor Cell Lines Using Retroviruses

      • Jodi E. D. Hecht, Youssef Jounaidi, David J. Waxman
      Pages 85-94

    4. 7 In Vitro Methods for Evaluation of P450-Based Anticancer Gene Therapy

      • Jodi E. D. Hecht, David J. Waxman
      Pages 95-105

    5. 8 Tumor Models for Evaluation of P450 Gene Therapy In Vivo

      • Jodi E. D. Hecht, Pamela Schreiber Schwartz, David J. Waxman
      Pages 107-118

  4. Experimental Approaces To Cancer Gene Therapy

    1. Front Matter

      Pages 1-436
      PDF

    2. 9 Intracellular Single-Chain Antibodies for Gene Therapy

      • Guadalupe Bilbao, Jesus Gomez-Navarro, Keizo Kazano, Juan Luis Contreras, David T. Curiel
      Pages 121-149

  5. Experimental Approaces To Cancer Gene Therapy

    1. Front Matter

      Pages 1-436
      PDF

    2. Inhibition of Cell Growth by Antisense Oligonucleotides Targeting the Growth-Related Protein Kinase c-raf

      • Doriano Fabbro, B. P. Monia, K.-H. Altmann, Thomas Geiger
      Pages 167-188

    3. IGF-1 Antisense Strategies for Cancer Treatment

      • Yue Xin Pan, Donald D. Anthony
      Pages 189-204

  6. Experimental Approaces To Cancer Gene Therapy

    1. Front Matter

      Pages 1-436
      PDF

    2. Anti-MDR1 Ribozyme Gene Therapy

      • Takao Ohnuma, Hiroyuki Kobayashi, Fu-Sheng Wang
      Pages 207-245

    3. Anti-c-erb1B2 Ribozyme for Gene Therapy of Breast Cancer

      • Toshiya Suzuki, Masami Bessho, Kevin J. Scanlon
      Pages 247-260
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About this book

Since the discovery of the molecular structure of genes and the unveiling of the molecular basis of numerous human diseases, scientists have been fas- nated with the possibility of treating certain diseases by transducing foreign DNA into the affected cells. Initially, it was proposed that the foreign DNA could either replace defective nonfunctional genes, or code for therapeutic proteins. This concept has evolved into the rapidly growing field of gene therapy. Even though surgery, radiotherapy, and chemotherapy are widely ava- able and routinely used for cancer treatment, these therapies fail to cure approximately 50 percent of cancer patients. Therefore, since it is a disease characterized by aberrant gene expression, cancer has been a target of gene therapy research since the inception of this treatment modality. Numerous cancer gene therapy strategies are currently being investigated, including gene replacement therapy, the regulation of gene expression to modulate immu- logical responses to tumors, the direct killing of tumor cells, and direct int- ference with tumor growth. In this context, gene transfer systems, tumor-specific expression vectors, and novel therapeutic genes have been extensively st- ied. All these strategies aim for the selective destruction of human malignant disease while circumventing the destruction of nonmalignant cells and tissues thereby minimizing toxicity to the patient.
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Reviews

"A wide variety of methods used to deliver genes to tumors are discussed, as are the type of genes used. This includes tumor vaccination, suicide genes, antisense genes, ribozymes, etc. The unique aspect of this book is the translational approach of explaining laboratory techniques as well as the application to patients. . .This book is well written and a translational resource for those interested in the rapidly advancing and novel field of gene therapy for malignancies."-Doody's Health Science Book Review Journal
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Editors and Affiliations

  • Max-Delbrück-Center for Molecular Medicine, Berlin, Germany

    Wolfgang Walther, Ulrike Stein

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Bibliographic Information

  • Book Title: Gene Therapy of Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Wolfgang Walther, Ulrike Stein

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592590861

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Softcover ISBN: 978-0-89603-843-1Published: 17 February 2000

  • eBook ISBN: 978-1-59259-086-5Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XVI, 645

  • Topics: Oncology

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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