Diagnostic and Therapeutic Antibodies

1. Front Matter

   Pages i-xiv

   PDF

2. Introduction

   1. The Antibody Molecule

      • Andrew J. T. George

      Pages 1-21

   2. Polyclonal and Monoclonal Antibodies

      • Mary A. Ritter

      Pages 23-34

   3. Engineering Antibody Molecules

      • Rakesh Verma, Ekaterini Boleti

      Pages 35-52

   4. Phage Display Technology

      • Michael Johns

      Pages 53-62

3. Antibodies in Medicine

   1. Prospects for the Application of Antibodies in Medicine

      • Herman Waldmann

      Pages 63-72

   2. Antibodies for Neoplastic Disease

      • Ian T. W. Matthews

      Pages 73-84

   3. The Application of Monoclonal Antibodies in the Treatment of Lymphoma

      • Martin J. Glennie, Jamie Honeychurch, Ruth R. French, Alison L. Tutt

      Pages 85-97

   4. Antibodies for Inflammatory Disease

      • Richard Smith

      Pages 99-114

   5. Antibodies for Inflammatory Disease

      • Peter C. Taylor

      Pages 115-139

   6. Antibodies for Transplantation

      • Denise L. Faustman

      Pages 141-156

   7. Antibody-Based Therapies in Infectious Diseases

      • H. Barbaros Oral, Cezmi A. Akdis

      Pages 157-178

   8. Antibodies in Nuclear Medicine

      • A. Michael Peters

      Pages 179-192

   9. Animal Models for Tumor Localization

      • Gail Rowlinson-Busza

      Pages 193-207

   10. Antibodies for Immunoassays

       • David J. Newman

       Pages 209-225

4. Ethics and Industry

   1. Intellectual Property

      • William M. Brown

      Pages 227-241

   2. From Laboratory to Clinic

      • Geoff Hale, Herman Waldmann

      Pages 243-266

5. Production and Purification

   1. Production of Monoclonal Antibodies

      • J’ona Freysd’ottir

      Pages 267-279

   2. Purification of Monoclonal Antibodies Using Protein A/G

      • Bridget Heelan

      Pages 281-288

   3. Preparation of Monoclonal Antibodies Using Ion Exchange Chromatography

      • Maureen Power

      Pages 289-293

Soon after the first description of monoclonal antibodies in 1976, there was enormous interest in the clinical application of antibodies, especially in the context of cancer. Antibodies appeared to offer the “magic bullet” that would allow the specific destruction of neoplastic cells. H- ever, many years’ effort resulted in very few cases of successful immu- therapy with antibodies. As a result there was a major backlash against antibody therapy, and the field lost a considerable amount of popularity. Fashion, in science as well as in other things, tends to be cyclical. Antibody-based therapy is once again attracting scientists and clinicians. There are several reasons for the renewed optimism; certainly the expe- ence of the last two decades has provided a wealth of information about problems associated with antibody therapy, and possible solutions to these problems. Recombinant antibody engineering has rejuvenated the field, allowing both the modification of antibodies to improve their in vivo pr- erties and the isolation of novel antibody molecules by such techniques as phage display. The results of recent clinical trials have demonstrated unequivocally the benefit of antibody therapy in a number of settings, and, finally, more careful consideration has been taken of the types of disease best treated using this approach.

"...a welcome addition to the clinician's medical library....written by experts in the field and cover a broad selection of issues, ranging from the basic structure of antibodies to intellectual property rights....Although the chapters can stand independently, the editors have made a major effort to cross-index them by giving references to related material in other chapters....this is a comprehensive book that should be useful to anyone interested in the use of antibodies for therapy, diagnosis, or laboratory investigation. As someone who is currently involved in the development of an antibody-based therapy, I found this book to be quite complete and well written. In many ways it can serve as a manual for the development and use of antibody reagents. The chapters are liberally sprinkled with welcome advice.... For clinicians, the book can provide a handy reference for the many types of antibody reagents available for clinical use, as well as information on serological assays. This book fills an important niche in the literature on antibody-based therapies, at a time when reagents are finding their way into clinical use in ever-increasing numbers." - Clinical Infectious Diseases

• Department of Immunology, Imperial College School of Medicine, Hammersmith Hospital, London, UK

  Andrew J. T. George

• Department of Pharmacology, University College, London, UK

  Catherine E. Urch

Policies and ethics