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  • Book
  • © 2000

DNA Repair Protocols

Prokaryotic Systems

Editors:

  1. Pat Vaughan

    1. HiberGen Ltd. and University College, Cork, Ireland

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 152)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    PDF

  2. Assays To Detect And Quantitate DNA Repair Activity

    1. Front Matter

      Pages 1-1
      PDF

    5. Detection of DNA Deoxyribophosphodiesterase Activity

      • Margarita Sandigursky, William A. Franklin
      Pages 39-48

    6. O 6-Alkylguanine-DNA Alkytransferase Assay

      • Amanda J. Watson, Geoffrey P. Margison
      Pages 49-61

    7. The E. coli Vsr Endonuclease

      • Claire G. Cupples, Georgina Macintyre
      Pages 63-73

    8. Coupling of DNA Helicase Function to DNA Strand Exchange Activity

      • Frank G. Harmon, Stephen C. Kowalczykowski
      Pages 75-89

    10. Holliday Junction Branch Migration and Resolution

      • Susana Gonzalez, James G. Wetmur
      Pages 107-118

  3. Use Of DNA Repair Enzymes As Tools In Molecular Biology

    1. Front Matter

      Pages 133-133
      PDF

    2. Flexible Genetic Engineering Using RecA Protein

      • Lance J. Ferrin
      Pages 135-147

    3. Interspecies Recombination and Mismatch Repair

      • Ivan Matic, François Taddei, Miroslav Radman
      Pages 149-157

    7. The Use of Resolvases T4 Endonuclease VII and T7 Endonuclease I in Mutation Detection

      • Jeffrey J. Babon, Matthew McKenzie, Richard G. H. Cotton
      Pages 187-199

  4. Back Matter

    Pages 201-209
    PDF
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About this book

When setting out to decide on the content of DNA Repair Protocols: Prokaryotic Systems, I was conscious of the need to portray the vast array of pathways and enzymatic activities that are part of the discipline of DNA repair. In addition to the classical DNA repair activities, I wanted to convey the significant interest that has been generated in recent years in the use of the proteins and repair systems as research tools, much like the use of restriction enzymes over the last few decades. Therefore, in addition to chapters deta- ing protocols for investigating specific repair activities, I have included s- eral chapters in this book on the applied use of DNA repair proteins and systems. The many years of research on bacterial DNA repair systems have allowed us to really understand the majority of DNA repair pathways in bac- rial cells. Building on this knowledge, research has lead to major advances in understanding mammalian DNA repair and uncovered its links to human d- ease, such as DNA mismatch repair and colon cancer, nucleotide excision repair and xeroderma pigmentosum, DNA helicase function in Bloom’s s- drome, and so on. Such have been the advances that Science magazine iden- fied the collective DNA repair systems as its “Molecule of the Year” in 1994.
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Reviews

"...must for any science library supporting a molecular biology program." - E-Streams

"An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher." - Lavoisier Librairie
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Editors and Affiliations

  • HiberGen Ltd. and University College, Cork, Ireland

    Pat Vaughan

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Bibliographic Information

  • Book Title: DNA Repair Protocols

  • Book Subtitle: Prokaryotic Systems

  • Editors: Pat Vaughan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590683

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Hardcover ISBN: 978-0-89603-643-7Published: 07 July 2000

  • Softcover ISBN: 978-1-61737-112-7Published: 10 November 2010

  • eBook ISBN: 978-1-59259-068-1Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 192

  • Topics: Cell Biology

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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