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  • Book
  • © 2000

Developmental Biology Protocols

Volume III

Editors:

  1. Rocky S. Tuan

    1. Thomas Jefferson University, Philadelphia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Cecilia W. Lo

    1. University of Pennsylvania, Philadelphia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 137)

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Table of contents (45 protocols)

  1. Front Matter

    Pages i-xxv
    PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      PDF

    2. Developmental Biology Protocols

      • Rocky S. Tuan, Cecilia W. Lo
      Pages 3-5

  3. Manipulation Of Developmental Gene Expression and Function

    1. Front Matter

      Pages 7-7
      PDF

    2. Ectopic Expression in Drosophila

      • Elizabeth L. Wilder
      Pages 9-14

    3. Clonal Analysis in the Examination of Gene Function in Drosophila

      • Jenny E. Rooke, Nicole A. Theodosiou, Tian Xu
      Pages 15-22

    4. Application of Antisense Oligodeoxynucleotides in Developing Chick Embryos

      • Peter G. Alexander, George L. Barnes, Rocky S. Tuan
      Pages 23-36

    5. Application of Functional Blocking Antibodies

      • Steven A. Oberlender, Rocky S. Tuan
      Pages 37-42

  4. Analysis of Gene Expression

    1. Front Matter

      Pages 43-43
      PDF

    3. Relative Reverse Transcription-Polymerase Chain Reaction

      • Troy A. Giambernardi, Robert J. Klebe
      Pages 51-58

    5. In Situ PCR Detection of HIV Expression in the Human Placenta

      • Asad U. Sheikh, Bruno M. Polliotti, Richard K. Miller
      Pages 75-86

    6. Gene Expression Analysis by In Situ Hybridization

      • Stefan Wawersik, Jonathan A. Epstein
      Pages 87-96

    7. Radio-Isotopic In Situ Hybridization on Tissue Sections

      • Antoon F. M. Moorman, Piet A. J. De Boer, Jan M. Ruijter, Jaco Hagoort, Diego Franco, Wouter H. Lamers
      Pages 97-115

    10. Multicolor Whole-Mount In Situ Hybridization

      • Giselbert Hauptmann, Thomas Gerster
      Pages 139-148

    11. Methods for Double Detection of Gene Expression

      • Ronald A. Conlon
      Pages 149-152

    12. Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins

      • Joseph C. Ayoob, Jean M. Sanger, Joseph W. Sanger
      Pages 153-157
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About this book

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in “experimental embry- ogy” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.
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Reviews

"We live in a time of rising expectations. Being a developmental biologist at the turn of the millenium demands expertise in embryological manipulation, viral gene expression, in situ hybridization, videomicroscopy, in vitro assays, transgenic embryo production, cell lineage analysis, PCR techniques, and computer-enhanced imaging technology. Rocky Tuan and Cecilia Lo have done the field (and each scientist within it) a wonderful service by collecting and editing the protocols of the masters of each echnique. More than 100 papers spanning these areas (and more) patiently lead one through each method, giving details on what brand of microscope slides to use, what centrifuge tubes work best, and where to purchase each piece of equipment. Copious notes provide details based on the laboratories' experiences of what works and what doesn't. Developmental Biology Protocols is a library in itself and will be essential for every laboratory of developmental biology. This
is the collection to get before your next grant application is due."-Scott Gilbert, Department of Biology, Swarthmore College, Swarthmore, PA

"The range of techniques, from viral vectors to imaging, and of organisms, from sea urchins to mammals, is comprehensive yet focused. This work should be an invaluable aid to those interested in all aspects of comparative development. This work is logically organized and presents a great opportunity for not only picking up a technique but also placing it in a logical context. Well done!" -Barbara B. Knowles, Director of Research, Jackson Labs, Bar Harbor, ME

"Among similar laboratory manuals Developmental Biology Protocols edited by Tuan and Lo impress by their breadth of coverage, timeliness, and scrupulous attention to detail. . .these protocols will be an invaluable aid to every geneticist and developmental biologist."-Davor Solter, Director of Developmental Biology, Max-Planck Institute, Freiburg, Germany
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Editors and Affiliations

  • Thomas Jefferson University, Philadelphia

    Rocky S. Tuan

  • University of Pennsylvania, Philadelphia

    Cecilia W. Lo

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Bibliographic Information

  • Book Title: Developmental Biology Protocols

  • Book Subtitle: Volume III

  • Editors: Rocky S. Tuan, Cecilia W. Lo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590667

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Hardcover ISBN: 978-0-89603-854-7Published: 21 January 2000

  • Softcover ISBN: 978-0-89603-576-8Published: 21 January 2000

  • eBook ISBN: 978-1-59259-066-7Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXVI, 522

  • Topics: Developmental Biology

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Buy it now

eBook USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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