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  • © 2001

Mycotoxin Protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 157)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
  2. General Techniques

    1. Front Matter

      Pages 1-48
    2. Mycotoxin Method Evaluation

      • Albert E. Pohland, Mary W. Trucksess
      Pages 3-10
    3. Sampling Techniques

      • Thomas B. Whitaker
      Pages 11-24
    4. 4 Preparation of Mycotoxin Standards

      • Stanley Nesheim, Michael E. Stack
      Pages 31-36
  3. Methods for Aspergillus Toxins

    1. Front Matter

      Pages 49-94
    2. Liquid Chromatographic Method for Aflatoxin M1 in Milk

      • Hans P. van Egmond, Sylviane Dragacci
      Pages 59-69
    3. Immunochemical Method for Cyclopiazonic Acid

      • Joe W. Dorner, Victor S. Sobolev, Wanjun Yu, Fun S. Chu
      Pages 71-80
    4. Immunochemical Method for Ochratoxin A

      • Ewald Usleber, Richard Dietrich, Elisabeth Schneider, Erwin Märtlbauer
      Pages 81-94
  4. Methods for Fusarium Toxins

    1. Front Matter

      Pages 95-170
    2. Chromatographic Methods forTrichothecenes

      • Takumi Yoshizawa
      Pages 115-129
    3. Chromatographic Method for the Determination of the Mycotoxin Moniliformin in Corn

      • Célestin Munimbazi, Lloyd B. Bullerman
      Pages 131-145
    4. Liquid Chromatographic Method for Fumonisins in Corn

      • Gordon S. Shephard
      Pages 147-158
    5. Enzyme-Linked Immunosorbent Assays of Zearalenone Using Polyclonal, Monoclonal and Recombinant Antibodies

      • Mi-Gyung Lee, Qiao-Ping Yuan, L. Patrick Hart, James J. Pestka
      Pages 159-170
  5. Methods for Other Fungal Toxins

    1. Front Matter

      Pages 171-234
    2. 15 Chromatographic Method for StachybotrysToxins

      • Simon F. Hinkley, Bruce B. Jarvis
      Pages 173-194

About this book

Mycotoxins produced by molds are common contaminants of many important crops, including wheat, corn, rice, and peanuts. Some mycotoxins are found in fruits and vegetables. These contaminants have a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. The occurrence of mycotoxins in foods is an unavoidable worldwide problem. About 80 countries have imposed regulatory limits to minimize human and animal exposure to mycotoxins. Regulatory limits, including international standards, have tremendous economic impact and must be developed using science-based risk assessments. The purpose of Mycotoxin Protocols is to provide the scientific and technological basis for analytical methods for use in obtaining the exposure data needed for risk assessments. Mycotoxin Protocols is divided into four sections, which are interc- nected. The first section: Chapters 1–5 describe the general techniques for mycotoxin analysis with emphasis on the importance of method validation based on statistical parameters; sampling procedures for collecting a sample as representative as possible of a bulk lot; the isolation of mycotoxins for use as analytical standards or for toxicological studies; the evaluation of purity and preparation of standards; and the detection and identification of impu- ties in isolated mycotoxins. Sections 2–4: Chapters 6–19 describe the most current chromatographic and immunochemical methods for studies on the major mycotoxins.

Reviews

"...many analysts will be attracted by this book in which assays for several mycotoxins are described." - MicroBiology Today

"After reading the complete book, I would like to congratulate the editors as well as all the authors for work well-done. This book is well-written and could be used both as a practical laboratory handbook as well as a reference book for research scientists. The authors have not only described the protocols in detail, but also reviewd different methodologies and provided notes of their own experience in some of the protocols. These notes are extremely useful for the readeres to understand the rationales, difficulties, steps to overcome the problems, alternatives for certain steps, precautions for certain steps as well as approaches to modify some steps/protocols for other related mycotoxins. " - Journal of Food Biochemistry

Editors and Affiliations

  • Food and Drug Administration, Washington, DC

    Mary W. Trucksess, Albert E. Pohland

Bibliographic Information

  • Book Title: Mycotoxin Protocols

  • Editors: Mary W. Trucksess, Albert E. Pohland

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590640

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2001

  • Hardcover ISBN: 978-0-89603-623-9Published: 06 September 2000

  • Softcover ISBN: 978-1-4899-3872-5Published: 17 August 2013

  • eBook ISBN: 978-1-59259-064-3Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 244

  • Topics: Pharmacology/Toxicology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access