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Extracellular Matrix Protocols

  • Book
  • © 2000

Overview

Editors:
  1. Charles H. Streuli

    1. The Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, University of Manchester, Manchester, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Michael E. Grant

    1. The Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, University of Manchester, Manchester, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 139)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Biochemistry of Extracellular Matrix

    1. Front Matter

      Pages xv-xv
      Download chapter PDF

    2. Semipermeabilized Cells to Study Procollagen Assembly

      • Richard R. Wilson, Neil J. Bulleid
      Pages 1-9

  3. Biophysics of Extracellular Matrix Proteins

    1. Front Matter

      Pages 49-49
      Download chapter PDF

    2. Quantitative Determination of Collagen Crosslinks

      • Trevor J. Sims, Nicholas C. Avery, Allen J. Bailey
      Pages 11-26

    3. Analysis of Laminin Structure and Function with Recombinant Glycoprotein Expressed in Insect Cells

      • Todd L. Mathus, Peter D. Yurchenco
      Pages 27-37

    4. Recombinant Collagen Trimers from Insect Cells and Yeast

      • Johanna Myllyharju
      Pages 39-48

    5. Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag

      • Neil Smyth, Uwe Odenthal, Barbara Merkl, Mats Paulsson
      Pages 49-57

    6. Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structural Study by Heteronuclear Nuclear Magnetic Resonance Spectroscopy

      • Jeremy R. Bright, Andrew R. Pickford, Jennifer R. Potts, Iain D. Campbell
      Pages 59-69

    7. Reconstitution of Functional Integrin into Phopsholipid Vesicles and Planar Lipid Bilayers

      • Eva-Maria Erb, Jürgen Engel
      Pages 71-82

    8. Confocal-FRAP Analysis of ECM Molecular Interactions

      • Timothy Hardingham, Philip Gribbon
      Pages 83-93

    9. Electron Cryomicroscopy of Fibrillar Collagens

      • Roger S. Meadows, David F. Holmes, Chris J. Gilpin, Karl E. Kadler
      Pages 95-109

    10. Atomic Force Microspopy Measurements of Intermolecular Binding Strength

      • Gradimir N. Misevic
      Pages 111-117

    11. ECM Macromolecules: Rotary Shadowing and Scanning Transmission Electron Microscopy

      • Michael J. Sherratt, Helen K. Graham, Cay M. Kielty, David F. Holmes
      Pages 119-132

  4. Molecular Biology of Extracellular Matrix Genes

    1. Front Matter

      Pages 133-133
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    2. Screening for Mutations in Cartilage ECM Genes

      • Michael D. Briggs
      Pages 133-145

    3. Tissue-Specific KO of ECM Proteins

      • Emilio Hirsch, Mara Brancaccio, Fiorella Altruda
      Pages 147-178

    4. Homologous Gene Targeting to Study ECM Assembly

      • Francesco Ramirez, Friedrich Laub, Hideaki Sumiyoshi
      Pages 179-185

    5. Enhancer Analysis of the α1(II) and α2(XI) Collagen Genes in Transfected Chondrocytes and Transgenic Mice

      • Noriyuki Tsumaki, Ying Liu, Yoshihiko Yamada, Paul Krebsbach
      Pages 187-195

    6. Retroviral Delivery of ECM Genes to Cells

      • Kyonggeun Yoon, Vitali Alexeev
      Pages 197-208
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About this book

It is now widely accepted that much of the dynamic function of cells and tissues is regulated from outside the cell by the extracellular matrix. In ad- tion to its conventional role in providing a scaffold for building tissues, the extracellular matrix acts as a directional highway for cellular movement and provides instructional information for promoting survival, proliferation, and differentiation. Indeed, the extracellular matrix is beginning to take a starring role in the choreography of cell and tissue function. The diverse roles of the extracellular matrix are reflected in its highly complicated structure, consisting of an ever increasing number of components. Yet the mechanisms of extracellular matrix assembly and how they influences cell behavior are only just beginning to be understood. In order to solve these problems new methodologies are, of necessity, being developed. Many of these technologies are highly sophisticated and are currently available only in a ha- ful of laboratories. However, we believe that they can readily be transported and established by other researchers. Thus, the purpose of Extracellular Matrix Protocols is to present some of these complicated techniques in a style that is relatively easy to reproduce.

Reviews

"This would be most useful to a lab researcher who had not previously been studying the role of the ECM in biological systems, and who wished to do so. Having all the practical protocols for preparing, assaying, and studying these important molecules in a small volume could be very useful for such a researcher."-Doody's Health Science Book Review Journal

Editors and Affiliations

  • The Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, University of Manchester, Manchester, UK

    Charles H. Streuli, Michael E. Grant

Bibliographic Information

  • Book Title: Extracellular Matrix Protocols

  • Editors: Charles H. Streuli, Michael E. Grant

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590632

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2000

  • eBook ISBN: 978-1-59259-063-6Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 370

  • Number of Illustrations: 50 b/w illustrations

  • Topics: Cell Biology

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