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Antigen Processing and Presentation Protocols

  • Book
  • © 2001

Overview

Editors:
  1. Joyce C. Solheim

    1. University of Nebraska Medical Center, Omaha

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Part of the book series: Methods in Molecular Biology (MIMB, volume 156)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Purification of 20S Proteasomes

    • Jill R. Beyette, Timothy Hubbell, John J. Monaco
    Pages 1-16

  3. Use of Proteasome Inhibitors to Examine Processing of Antigens for Major Histocompatibility Complex Class I Presentation

    • Luis C. Antón, Jack R. Bennink, Jonathan W. Yewdell
    Pages 17-31

  4. Tracing the Route Taken by Peptides and Major Histocompatibility Complex Class I Molecules in Presentation of Exogenous Antigens

    • Christopher C. Norbury
    Pages 33-48

  5. Functional Analysis of Antigen Processing and Major Histocompatibility Complex Class II-Restricted Presentation

    • John D. Lich, Janice S. Blum
    Pages 49-56

  6. Quantitation of Major Histocompatibility Complex Antigen Endocytosis Using Capture ELISA

    • Diane N. Turvy, Janice S. Blum
    Pages 57-65

  7. In Vitro Generation of Functional Human and Murine Dendritic Cells

    • SeemaS. Ahuja
    Pages 67-77

  8. Genetic Engineering of Dendritic Cells Using Retrovirus-Based Gene Transfer Techniques

    • Seema S. Ahuja
    Pages 79-87

  9. Use of Vaccinia Virus Expression Vectors to Investigate Antigen Processing and Presentation

    • E. John Wherry, Deepa Rajagopal, Laurence C. Eisenlohr
    Pages 89-109

  10. Sindbis Virus-Based Vectors for the Study of Class I Antigen Presentation In Vitro and In Vivo

    • John M. Polo, Cornelia C. Bergmann
    Pages 111-128

  11. Precision Genotyping of Human Leukocyte Antigen-A, -B and -C Loci Via Direct DNA Sequencing

    • Mary Ellexson-Turner, David Sidebottom, Sean Turner, Tara Bennett, William H. Hildebrand
    Pages 129-142

  12. Photolabeling the Transporter Associated with Antigen Processing

    • Vashti G. Lacaille, Matthew J. Androlewicz
    Pages 143-151

  13. Application of One-Dimensional Isoelectric Focusing to Separate Different Major Histocompatibility Complex Class I Alleles and Determine Their Allelic Interactions with Transporter Associated with Antigen Processing (TAP)

    • Anne Neisig, Jacques Neefjes
    Pages 153-163

  14. Analysis of Major Histocompatibility Complex Class I Interactions with Endoplasmic Reticulum Proteins

    • Heth R. Turnquist, Joyce C. Solheim
    Pages 165-173

  15. Methods of Purification of Heat Shock Protein-Peptide Complexes for Use as Vaccines Against Cancers and Infectious Diseases

    • Pramod K. Srivastava, Navdeep S. Jaikaria
    Pages 175-186

  16. Random Peptide Libraries

    • James Stevens, Geoffrey W. Butcher
    Pages 187-199

  17. Neural Network Method for Predicting Peptides that Bind Major Histocompatibility Complex Molecules

    • Kamalakar Gulukota, Charles DeLisi
    Pages 201-209

  18. Analysis of T-Cell Response Using Altered Peptide Ligands

    • Florence Boisgérault, Natalie Anosova, Eugenia V. Fedoseyeva, Robert C. Tam, Gilles Benichou
    Pages 211-218

  19. Assays of Thymic Selection Fetal Thymus Organ Culture and In Vitro Thymocyte Dulling Assay

    • Kristin A. Hogquist
    Pages 219-232

  20. Protocol for Diversion of a CD4+Response to theT-Helper 2 Cell Pathway

    • Francesca Galbiati, Luciano Adorini
    Pages 233-244
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About this book

Antigen processing and presentation, as a field, explores a broad range of protein interactions and functions, both intracellular (in the cytoplasm and in the endoplasmic reticulum) and at the cell surface (between T cells and MHC molecules). To investigate such a diverse array, it is necessary that biochemical, cell biology, and immunological techniques all be employed. The purpose of Antigen Processing and Presentation Protocols is therefore to detail the most up-to-date techniques being used in this burgeoning field. Such techniques include those used to question how MHC-binding peptides are generated, to test how peptides are delivered to MHC molecules, to analyze MHC peptide-binding patterns, and to assay the T-cell response to MHC/peptide. Antigen Processing and Presentation Protocols should aid both those new and those experienced in this area of research in extending the questions that can be asked and answered by the application of these current methods. For editorial assistance, I would like to thank Angela Beninga and Rachael Turnquist.

Editors and Affiliations

  • University of Nebraska Medical Center, Omaha

    Joyce C. Solheim

Bibliographic Information

  • Book Title: Antigen Processing and Presentation Protocols

  • Editors: Joyce C. Solheim

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590624

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • Hardcover ISBN: 978-0-89603-745-8Published: 15 September 2000

  • Softcover ISBN: 978-1-61737-166-0Published: 10 November 2010

  • eBook ISBN: 978-1-59259-062-9Published: 26 October 2007

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: IX, 270

  • Topics: Immunology

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