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Chaperonin Protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 140)

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Table of contents (22 protocols)

  1. Front Matter

    Pages I-X
  2. Purification of Archaeal Chaperonin from Sulfolobus shibatae

    • Elsie Quaite-Randall, Andrzej Joachimiak
    Pages 1-14
  3. Purification of Hsp60 from Thermus thermophilus

    • Elsie Quaite-Randall, Andrzej Joachimiak
    Pages 15-27
  4. Purification of GroEL from an Overproducing E. coliStrain

    • Elsie Quaite-Randall, Andrzej Joachimiak
    Pages 29-39
  5. Purification of GroES from an Overproducing E. coliStrain

    • Elsie Quaite-Randall, Andrzej Joachimiak
    Pages 41-49
  6. Determination of Chaperonin Activity In Vivo

    • Saskia M. van der Vies, Peter A. Lund
    Pages 75-96
  7. Prevention of Rhodanese Aggregation by the Chaperonin GroEL

    • Frank Weber, Manajit Hayer-Hartl
    Pages 111-115
  8. Assay of Malate Dehydrogenase

    • Manajit Hayer-Hartl
    Pages 127-132
  9. Assay of Chaperonin-Assisted Refolding of Citrate Synthase

    • N. Kalaya Steede, Stacy L. Temkin, Samuel J. Landry
    Pages 133-138
  10. Purification of Yeast Mitochondrial Hsp60

    • Yves Dubaquié, Renate Looser, Sabine Rospert
    Pages 139-143
  11. Preparation of Recombinant Human Hsp10

    • N. Kalaya Steede, Jesse J. Guidry, Samuel J. Landry
    Pages 145-151
  12. Purification of the Cytosolic ChaperoninTRiC from Bovine Testis

    • Raul G. Ferreyra, Judith Frydman
    Pages 153-160
  13. Monitoring Actin Folding

    • Vanita Thulasiraman, Raul G. Ferreyra, Judith Frydman
    Pages 161-167
  14. Folding Assays

    • Vanitha Thulasiraman, Raul G. Ferreyra, Judith Frydman
    Pages 169-177

About this book

In Chaperonin Protocols, Christine Schneider has assembled a unique collection of readily reproducible protocols for the study of chaperonins, intracellular proteins critical to many biological processes. Written by experienced investigators who have successfully honed their methods to a fineness, the protocols focus on the purification of chaperonins from different species along with their corresponding cofactors, and on chaperonin activity assays for in vivo as well as in vitro work. Many activity assays are given for GroEL, which can also be applied to mitochrondrial Hsp60. There are also assays for the eukaryotic chaperonin TRiC and handy methods-for example, one for preparing labeled probes-that can be used for various purposes and prove helpful in numerous different procedures.
Critically important to a greater understanding of such disorders as cystic fibrosis, Alzheimer's disease, and BSE, Chaperonin Protocols offers both novice and experienced investigators fast access to today's best and most productive chaperonin methods, all explained in step-by-step detail to ensure robust and reproducible results.

Reviews

"This book is highly recommended for academic libraries and for those special or industrial libraries that support a program in microbiology. Because the clear and logical approach to each protocol can serve as a model experiment that is easily reproduced, this book would be an especially valuable tool for students."-E-Streams (Electronic Reviews of Science and Technology References)

"...an invaluable overview of two main aspects of chaperonin research-purification of chaperonins and their cofactors and assays to monitor folding activity. ...of excellent chapters on more diverse chaperonins, including those from Archaea and the specialized, eukaryotic TRiC complex....The book provides a wealth of explicitly detailed protocols (together with useful troubleshooting notes) for all those working in the field of chaperonins and protein folding or misfolding."...Microbiology Today

Editors and Affiliations

  • Max-Planck Institute for Biochemistry, Martinsried, Germany

    Christine Schneider

Bibliographic Information

  • Book Title: Chaperonin Protocols

  • Editors: Christine Schneider

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590616

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Hardcover ISBN: 978-0-89603-739-7Published: 19 January 2000

  • Softcover ISBN: 978-1-61737-163-9Published: 10 November 2010

  • eBook ISBN: 978-1-59259-061-2Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 212

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access