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  • Book
  • © 2000

Stress Response

Methods and Protocols

Editors:

  1. John M. Walker
    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Stephen M. Keyse

    1. ICRF Laboratories, University of Dundee, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 99)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xvi
    PDF

  2. Detection and Assay of Stress-Induced Damage

    1. Front Matter

      Pages 1-1
      PDF

    3. Determination of Carbonyl Groups in Oxidized Proteins

      • Rodney L. Levine, Nancy Wehr, Joy A. Williams, Earl R. Stadtman, Emily Shacter
      Pages 15-24

    4. Quantitation of 4-Hydroxynonenal Protein Adducts

      • Koji Uchida, Earl R. Stadtman
      Pages 25-34

    6. The Measurement of Protein Degradation in Response to Oxidative Stress

      • Thomas Reinheckel, Tilman Grune, Kelvin J. A. Davies
      Pages 49-60

  3. The Activation of Signal Transduction by Cellular Stress

    1. Front Matter

      Pages 61-61
      PDF

    2. Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress

      • D. Grahame Hardie, Ian P. Salt, Stephen P. Davies
      Pages 63-74

    5. Expression and Assay of Recombinant ATM

      • Yael Ziv, Sharon Banin, Dae-Sik Lim, Christine E. Canman, Michael B. Kastan, Yosef Shiloh
      Pages 99-108

    6. Detection and Purification of a Multiprotein Kinase Complex from Mammalian Cells

      • Frank Mercurio, David B. Young, Anthony M. Manning
      Pages 109-125

    9. Use of Kinase Inhibitors to Dissect Signaling Pathways

      • Ana Cuenda, Dario R. Alessi
      Pages 161-175

    10. The Development and Use of Phospho-Specific Antibodies to Study Protein Phosphorylation

      • Jeremy P. Blaydes, Borek Vojtesek, Graham B. Bloomberg, Ted R. Hupp
      Pages 177-189

  4. The Analysis of Stress-Induced Gene Expression

    1. Front Matter

      Pages 202-202
      PDF

    2. Assaying NF-KB and AP-1 DNA-Binding and Transcriptional Activity

      • Judith M. Mueller, Heike L. Pahl
      Pages 205-216
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About this book

Mammalian cells have evolved a complex multicomponent machinery that enables them to sense and respond to a wide variety of potentially toxic agents present in their environment. These stress responses are often associated with an increased cellular capacity to tolerate normally lethal levels of an insult. The realization that the mammalian stress response may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer, has led to an explosion of interest in this research area. Stress Response: Methods and Protocols brings together a diverse array of practical methodologies that may be employed to address various aspects of the response of mammalian cells to environmental stress. The p- tocols are carefully described by authors who have both devised and succe- fully employed them, and they represent a mixture not only of well-established techniques, but also new technologies at the leading edge of research. The areas covered include the detection and assay of stress-induced damage, the acti- tion of signal transduction pathways, stress-inducible gene expression, and stress protein function. Although no volume of this size can be comprehensive and the topics covered reflect a personal choice, it is hoped that it will prove of subst- tial interest and use to a wide range of research workers in the field.
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Reviews

"This handbook will be very helpful in this respect for everybody interested in studies on the effects of stress at the cellular level using biochemical and molecular biology methods." -Cell Biology International
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Editors and Affiliations

  • ICRF Laboratories, University of Dundee, UK

    Stephen M. Keyse

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Bibliographic Information

  • Book Title: Stress Response

  • Book Subtitle: Methods and Protocols

  • Editors: John M. Walker, Stephen M. Keyse

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590543

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Hardcover ISBN: 978-0-89603-611-6Published: 19 May 2000

  • Softcover ISBN: 978-1-61737-097-7Published: 10 November 2010

  • eBook ISBN: 978-1-59259-054-4Published: 26 October 2007

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 488

  • Topics: Cell Biology

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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