Bacterial Toxins

1. Front Matter

   Pages i-xi

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2. Bacterial Protein Toxins

   • Joseph E. Alouf

   Pages 1-26

3. Purification of Clostridium botulinum Type A Neurotoxin

   • Carl J. Malizio, Michael C. Goodnough, Eric A. Johnson

   Pages 27-39

4. Shiga Toxins

   • David W. K. Acheson, Anne V. Kane, Gerald T. Keusch

   Pages 41-63

5. Isolation and Detection of Microcystins and Nodularins, Cyanobacterial Peptide Hepatotoxins

   • Jussi Meriluoto, Linda Lawton, Ken-ichi Harada

   Pages 65-87

6. Genetic Construction, Expression, and Characterization of Diphtheria Toxin-Based Growth Factor Fusion Proteins

   • Johanna C. vanderSpek, John R. Murphy

   Pages 89-99

7. Structure-Function Analysis of Cysteine-Engineered Entomopathogenic Toxins

   • Jean-Louis Schwartz, Luke Masson

   Pages 101-113

8. Use of Fourier-Transformed Infrared Spectroscopy for Secondary Structure Determination of Staphylococcal Pore-Forming Toxins

   • Gianfranco Menestrina

   Pages 115-132

9. The Use of Fluorescence Resonance Energy Transfer to Detect Conformational Changes in Protein Toxins

   • William D. Picking

   Pages 133-146

10. Site-Directed Spin Labeling of Proteins

    • Kyoung Joon Oh, Christian Altenbach, R. John Collier, Wayne L. Hubbell

    Pages 147-169

11. Characterization of Molecular Properties of Pore-Forming Toxins with Planar Lipid Bilayers

    • Mauro Dalla Serra, Gianfranco Menestrina

    Pages 171-188

12. Determination of Affinity and Kinetic Rate Constants Using Surface Plasmon Resonance

    • Luke Masson, Alberto Mazza, Gregory De Crescenzo

    Pages 189-201

13. ADP-Ribosylation of α-Gi Proteins by Pertussis Toxin

    • Lars von Olleschik-Elbheim, Ali el Bayâ, M Alexander Schmidt

    Pages 203-217

14. Phage Libraries for Generation of Anti-Botulinum scFv Antibodies

    • Peter Amersdorfer, James D. Marks

    Pages 219-240

15. T-Cell Cytotoxicity Assays for Studying the Functional Interaction Between the Superantigen Staphylococcal Enterotoxin A and T-Cell Receptors

    • Alexander Rosendahl, Karin Kristensson, Kristian Riesbeck, Mikael Dohlsten

    Pages 241-257

16. In Vitro Physiological Studies on Clostridial Neurotoxins

    • Bernard Poulain, Marie-France Bader, Jordi Molgó

    Pages 259-286

17. The Biology of Endotoxin

    • Volker T. El-Samalouti, Lutz Hamann, Hans Dieter Flad, Artur J. Ulmer

    Pages 287-309

18. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Lipopolysaccharides

    • Buko Lindner

    Pages 311-325

19. Applications of Combined Capillary Electrophoresis-Electrospray Mass Spectrometry in the Characterization of Short-Chain Lipopolysaccharides

    • Pierre Thibault, James C. Richards

    Pages 327-344

20. Deacylation of Lipopolysaccharides and Isolation of Oligosaccharide Phosphates

    • Otto Holst

    Pages 345-353

The interest of investigators across a broad spectrum of scientific dis- plines has been steadily stimulated by the field of bacterial toxin research, an area that makes use of a large variety of biological, chemical, physicochemical, and medically oriented approaches. Researchers studying bacterial toxins need to be acquainted with all these disciplines in order to work effectively in the field. To date, there has been no published collection offering detailed descr- tions of the techniques and methods needed by researchers operating across the field’sdiverse areas. The present volume Bacterial Toxins: Methods and Pro- cols, is intended to fill this gap. Bacterial Toxins: Methods and Protocols consists of two sections: one on protein toxins (15 chapters) and one on endotoxins (5 chapters). Each s- tion is introduced by an overview article (Chapters 1 and 16). The protocols collected represent state-of-the-art techniques that each have high impact on future bacterial toxin research. All methods are described by authors who have regularly been using the protocol in their own laboratories. Included in each chapter is a brief introduction to the method being described.

"The editor has put together an outstanding text on the current protocols used in the study of protein toxins. The caliber of the contributors is outstanding and represents an international perspective. While the editor does not present the text as an exhaustive discussion of microbial toxins, there is a focus on those areas where there is current and active research such as toxin-receptor kinetics, the use of fluorescence resonance energy transfer to detect structural changes in protein toxins, and CE-ESMS of short-chain lipopolysaccharides in Haemophilus influenzae. The organization and content level of this manual makes it a highly desirable addition to either a personal or a departmental library. "-Doody's Health Science Book Review Journal

• Research Center Borstel, Germany

  Otto Holst

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