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  • Book
  • © 2001

Cytoskeleton Methods and Protocols

Editors:

  1. Ray H. Gavin

    1. Department of Biology, Brooklyn College, The City University of New York

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 161)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Identification of Cytoskeleton Proteins

    1. Front Matter

      Pages 1-1
      PDF

    4. Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena

      • Gangadhara Sailaja, Leslie M. Lincoln, Jifan Chen, David J. Asai
      Pages 17-27

  3. Microscopy Applications

    1. Front Matter

      Pages 29-29
      PDF

    3. Computer-Assisted Systems for the Analysis of Amoeboid Cell Motility

      • David R. Soil, Deborah Wessels, Edward Voss, Olof Johnson
      Pages 45-58

    4. Evaluation of Individual-Cell Motility

      • Peter S. Walmod, Rasmus Hartmann-Petersen, Anton Berezin, Søren Prag, Vladislav V. Kiselyov, Vladimir Berezin et al.
      Pages 59-83

    5. Evaluation of Cell Morphology

      • Eugene A. Lepekhin, Peter S. Walmod, Anton Berezin, Vladimir Berezin, Elisabeth Bock
      Pages 85-100

    6. A Quantitative Assay for Measurement of Chemokinesis in Tetrahymena

      • Uffe Koppelhus, Per Hellung-Larsen, Vagn Leick
      Pages 101-106

  4. Reagents for Studying Cytoskeleton Protein Function

    1. Front Matter

      Pages 107-107
      PDF

    2. Jasplakinolide

      • Andreas Holzinger
      Pages 109-120

  5. Cytoskeleton Dynamics

    1. Front Matter

      Pages 149-149
      PDF

    2. Studying Cytoskeletal Dynamics in Living Cells Using Green Fluorescent Protein

      • Yisang Yoon, Kelly R. Pitts, Mark A. McNiven
      Pages 151-163

    3. Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics

      • Hong Cao, Heather Thompson, Eugene W. Krueger, Mark McNiven
      Pages 165-187
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About this book

Over the past two decades experimental studies have solidified the int- pretation of the cytoskeleton as a highly dynamic network of microtubules, actin microfilaments, intermediate filaments, and myosin filaments. Rather than a network of disparate fibers, these polymers are often interconnected and display synergy, which is the combined action of two or more cytoskeletal polymers to achieve a specific cellular structure or function. Cross-commu- cation among cytoskeletal polymers is thought to be achieved through cytoskeletal polymer accessory proteins and molecular motors that bind two or more cytoskeletal polymers. Development of the modern concept of the cytoskeleton is a direct o- growth of advances in experimental tools and reagents that are available to cell and molecular biologists. Technological advances and refinements in cell imaging have made it possible to selectively image a single cytoskeletal po- mer and monitor its dynamics through the use of fluorescence probes in vitro and in vivo. Two decades ago, cytoskeletal research was limited to a few perturbation reagents that included colchicine and cytochalasin. Today, the perturbation arsenal has expanded to a highly selective group of reagents that includes Taxol, nocodazole, benomyl, latrunculin, jasplakinolide, and such endogenous proteins as gelsolin. These reagents enable the investigator to selectively perturb or destroy a cytoskeletal polymer while leaving other cytoskeletal polymers intact. Site-specific monoclonal antibodies that target a specific cytoskeletal polymer have proven to be highly selective affinity tools for cytoskeletal research.
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Reviews

"The authors of the chapters are highly experienced and widely recognized as experts in their fields...The volume is one that the libraries of many universities and research institutes will want to own as a resource for their cytoskeleton researchers." - TRENDS in Cell Biology

"The material and procedures sections of the chapters are generally well detailed such that one with little previous experience might be able to carry out the procedure or adapt it to one's own particular system. The chapters include helpful hints on the techniques which are often not found in published articles. As a microscopist, I was impressed by the simple but elegant procedure of placing a finger print on the corner of a cover slip in order to determine the plane of focus in video-enhanced microscopy, presented by George Langford in Chapter 4. It is often just those very kinds of things that can mean the difference between success and failure, or at least help to reduce the frustration level of a first timer." - Microscopy and Microanalysis

"As a cell biologist, I was impressed by the chapters dealing with the study of cytoskeletal dynamics in living cells using green fluorescent protein and the study of organelle transport using chromatophores as tools you got the true impression you can do it thanks to the step-by-step protocols, you feel you cannot be wrong and I personally worked out one clear experiment. I will do on my own on mouse oocytes. I think the whole scientific community has to thank Dr. Gavin for providing us with such a helpful book." - European Journal of Histochemistry

"Cytoskeleton: Methods and Protocols emphasizes an integrative approach and is intended for novice and advanced researchers. It is highly recommended for libraries serving researchers interested in cytoskeleton structure and function and related areas of cell biology. " - E-STREAMS
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Editors and Affiliations

  • Department of Biology, Brooklyn College, The City University of New York

    Ray H. Gavin

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Bibliographic Information

  • Book Title: Cytoskeleton Methods and Protocols

  • Editors: Ray H. Gavin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590519

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • eBook ISBN: 978-1-59259-051-3Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 288

  • Topics: Cell Biology

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