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  • Book
  • © 2000

Calpain Methods and Protocols

Editors:

  1. John S. Elce

    1. Department of Biochemistry, Queen’s University, Kingston, Canada

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 144)

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Table of contents (37 protocols)

  1. Front Matter

    Pages i-xviii
    PDF

  2. Purification of Components of the Calpain System

    1. Front Matter

      Pages 1-105
      PDF

    2. Purification of µ- and m-Calpain and Calpastatin from Animal Tissues

      • Valery F Thompson, Darrel E Goll
      Pages 1-16

    9. Drosophila Cal pains

      • Gàspàr Jékely, Marianna Pintér, Peter Friedrich
      Pages 67-74

    12. Preparation of Calpastatin Samples for Western Blotting

      • Masatoshi Maki, Kiyotaka Hitomi
      Pages 95-98

    13. Isolation and Characterization of Calpain Activator Protein from Bovine Brain

      • Edon Melloni, Mauro Michetti, Franca Salamino, Roberto Minafra, Bianca Sparatore, Sandro Pontremoli
      Pages 99-105

  3. Assays of Calpain

    1. Front Matter

      Pages 109-169
      PDF

    2. Calpain Zymography with Casein or Fluorescein Isothiocyanate Casein

      • J. Simon C. Arthur, Donald L. Mykles
      Pages 109-116

    3. Casein Zymogram Assessment of μ-Calpain and m-Calpain Activity After Traumatic Brain Injury in the Rat In Vivo

      • Xiurong Zhao, Jennifer K. Newcomb, Brian R. Pike, Ronald L. Hayes
      Pages 117-120

    4. Fluorescence Measurements of Ca2+ Binding to Domain VI of Calpain

      • J. Simon C. Arthur, John S. Elce
      Pages 121-127

    5. Kinetic Analysis of Human μ-Calpain Autolysis

      • Peter Tompa, Peter Friedrich
      Pages 129-136

    6. A Sensitive and Continuous Fluorometric Activity Assay Using a Natural Substrate

      • Peter Tompa, Éva Schád, Peter Friedrich
      Pages 137-141
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About this book

The purpose of Calpain Methods and Protocols is quite straightf- ward: it is to present the actual experimental methods used in many different laboratories for the study of calpain. It will provide the vital experimental detail, and the discussion of possible pitfalls, for which the standard journals no longer provide space. This will make it as easy as possible for investi- tors interested in calpain to adopt established methods without repeating old mistakes, and to adapt and apply these methods in novel approaches to the many outstanding calpain questions. These questions range from purely biochemical problems of protein structure and enzyme regulation at the molecular level, through large areas of cell biology, to applied and clinical aspects of calpain function in human d- ease. Within this panoply of topics, a wide range of investigators will find many fascinating and as yet unanswered questions about calpain. Calpain Methods and Protocols will provide instant access to many essential te- niques, while saving them the time and effort involved in developing a new method. In addition to questions relating to the normal physiological roles of the calpains, there is considerable evidence that inappropriate calpain activity may have pathological effects in many tissues, for example, following ischemia. This provides a major stimulus for the development of specific calpain inhi- tors for therapeutic purposes, and for the development of methods to evaluate such inhibitors.
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Reviews

"This book collects contributions from essentially all leading specialists in the area and the chapters are constructed in a way that should make it a useful bench-top companion. The book is good value for money: 36 chapters for $89 should make it an all but mandatory acquisition for libraries, and especially laboratories having programs on cell biology and cell pathology." -Cell Biology International
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Editors and Affiliations

  • Department of Biochemistry, Queen’s University, Kingston, Canada

    John S. Elce

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Bibliographic Information

  • Book Title: Calpain Methods and Protocols

  • Editors: John S. Elce

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592590500

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Hardcover ISBN: 978-0-89603-632-1Published: 21 February 2000

  • Softcover ISBN: 978-1-61737-105-9Published: 10 November 2010

  • eBook ISBN: 978-1-59259-050-6Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 342

  • Topics: Neurosciences

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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