Overview
- Editors:
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Ian M. Clark
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University of East Anglia, Norwich, UK
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Table of contents (33 protocols)
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MMPs and TIMPs—An Overview of the Field
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- J. Frederick Woessner Jr.
Pages 1-23
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- Gloria Velasco, Carlos López-OtÃn
Pages 25-44
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- Wolfram Bode, Klaus Maskos
Pages 45-77
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- Lisa M. Coussens, Steven D. Shapiro, Paul D. Soloway, Zena Werb
Pages 149-179
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Expression and Purification of MMPs and TIMPs
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Front Matter
Pages 181-304
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- Karen M.-L. Yeow, Blaine W. Phillips, P. Paul Beaudry, Kevin J. Leco, Gillian Murphy, Dylan R. Edwards
Pages 181-189
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- L. Jack Windsor, Darin L. Steele
Pages 191-205
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- Rüdiger Vallon, Peter Angel
Pages 207-218
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- Michael J. Butler, Marie-Pia d’Ortho, Susan J. Atkinson
Pages 239-255
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- Ken-ichi Shimokawa, Hideaki Nagase
Pages 275-304
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Detection of MMPs and TIMPs
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Front Matter
Pages 305-375
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- Howard Wong, Huong Muzik, Lori L. Groft, Marc A. Lafleur, Charles Matouk, Peter A. Forsyth et al.
Pages 305-320
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About this book
Research in the matrix metalloproteinase field began with the demonstration by Gross and Lapière, in 1962, that resorbing tadpole tail expressed an enzyme that could degrade collagen gels. These humble beginnings have led us to the elucidation of around twenty distinct vertebrate MMPs, along with a variety of homologs from such diverse organisms as sea urchin, plants, nematode worm, and bacteria. This, coupled with four known specific inhibitors of MMPs, the TIMPs, gives a complex picture. Part I of Matrix Metalloproteinase Protocols provides the reader with a selective overview of the MMP arena, and a chance to come to grips with where the field has been, where it is, and where it is going. I hope that this complements all of the methodology that comes later. Part II presents the reader with a diverse set of methods for the expression and purification of MMPs and TIMPs, bringing together the long and often hard-earned experience of a number of researchers. Part III allows the reader to detect MMPs and TIMPs at both the protein and mRNA level, whereas Part IV gives the ability to assay MMP and TIMP activities in a wide variety of circumstances.
Reviews
"The editor, Ian M. Clark, has done an excellent job in putting together a collection of articles that span what is now a fairly wide field. ...the content and writing are of a high standard...In chapter 3, covering structural studies and contributed by Wolfram Bode and Klaus Maskos, the authors point out the absence of specific discussions of three-dimensional structures amongst the plethora of general reviews on MMPs and TIMPs. Their contribution expertly fills that void....Indeed the 'Notes' section, at the end of each chapter, is where the benefit of much, often hard-earned, experience is to be reaped and is a valuable addition in almost every case. The last two sections, between them, cover detection of MMp and TIMP expression at both RNA and protein levels, and actively assays. Hore, particular mention must be made of the chapter dealing with zymography and reverse zymography in which Susan Hawkes and coauthors have carefully explained this technique. Their use of figures with detailed interpretation is really excellent and alone could justify the purchase of this book for many labs....this book is recommended as a reference text for all labs engaged in MMP research. There is enough variety for established research labs to find some new methods, and, for newcomers to the field, it should make working with MMPs and their inhibitors a very straightforward prospect." - TRENDS in Cell Biology
Editors and Affiliations
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University of East Anglia, Norwich, UK
Ian M. Clark
