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  • © 2009

Plant Genomics

Methods and Protocols

  • Provides insight into use of model plant species for genomics
  • Includes exceptional descriptions of bioinformatics tools available to plant genomics
  • Offers a clear description of mapping gene expression patterns in plants
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 513)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
  2. Role of Model Plant Species

    • Richard Flavell
    Pages 1-18
  3. New Technologies for Ultra-High Throughput Genotyping in Plants

    • Nikki Appleby, David Edwards, Jacqueline Batley
    Pages 19-39
  4. Genetic Maps and the Use of Synteny

    • Chris Duran, David Edwards, Jacqueline Batley
    Pages 41-55
  5. A Simple TAE-Based Method to Generate Large Insert BAC Libraries from Plant Species

    • Bu-Jun Shi, J. Perry Gustafson, Peter Langridge
    Pages 57-80
  6. Transcript Profiling and Expression Level Mapping

    • Elena Potokina, Arnis Druka, Michael J. Kearsey
    Pages 81-92
  7. Methods for Functional Proteomic Analyses

    • Christof Rampitsch, Natalia V. Bykova
    Pages 93-110
  8. Stable Transformation of Plants

    • Huw D. Jones, Caroline A. Sparks
    Pages 111-130
  9. Transient Transformation of Plants

    • Huw D. Jones, Angela Doherty, Caroline A. Sparks
    Pages 131-152
  10. Bridging the Gene-to-Function Knowledge Gap Through Functional Genomics

    • Stephen J. Robinson, Isobel A. P. Parkin
    Pages 153-173
  11. Heterologous and Cell-Free Protein Expression Systems

    • Naser Farrokhi, Maria Hrmova, Rachel A. Burton, Geoffrey B. Fincher
    Pages 175-198
  12. In situ Analysis of Gene Expression in Plants

    • Sinéad Drea, Paul Derbyshire, Rachil Koumproglou, Liam Dolan, John H. Doonan, Peter Shaw
    Pages 229-242
  13. Plant and Crop Databases

    • David E. Matthews, Gerard R. Lazo, Olin D. Anderson
    Pages 243-262
  14. Plant Genome Annotation Methods

    • Shu Ouyang, Françoise Thibaud-Nissen, Kevin L. Childs, Wei Zhu, C. Robin Buell
    Pages 263-282
  15. Molecular Plant Breeding: Methodology and Achievements

    • Rajeev K. Varshney, Dave A. Hoisington, Spurthi N. Nayak, Andreas Graner
    Pages 283-304
  16. Practical Delivery of Genes to the Marketplace

    • David A. Fischhoff, Molly N. Cline
    Pages 305-320
  17. Genome Sequencing Approaches and Successes

    • Michael Imelfort, Jacqueline Batley, Sean Grimmond, David Edwards
    Pages 345-358
  18. Back Matter

    Pages 359-362

About this book

Plant genomics is a growing and constantly evolving field of study, one which has gained much ground in past years through the development of advanced research and data management tools. In Plant Genomics: Methods and Protocols, expert researchers explore the current issues and methodologies of this expanding field, specifically addressing areas of gene discovery and the functional analysis of genes with a focus on the primary tools and sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling. Chapters employ exciting new methods to investigate molecular plant breeding technology and gene functional analysis via transformation, mutation, protein function, and gene expression. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and innovative, Plant Genomics: Methods and Protocols is an essential guide for all plant scientists who are interested in further studies in the area of genomics research.

Reviews

From the reviews:

“The book is latest volume in the series ‘Methods in Molecular Biology’ … . The volume ‘Plant Genomics. Methods and Protocols’ concerns a wide spectrum of problems, some of them outside of the main scope of the field … . In summary, ‘Plant Genomics. Methods and Protocols’ can be recommended as a valuable source of timely information for students, researchers and breeders.” (Paweł Sowiński, Acta Biochimica Polonica, December, 2009)

“This book is part of the very successful Methods in Molecular Biology series that provides research protocols and methodologies for biological scientists. … the book does succeed in covering a wide range of topics and will therefore be a useful addition to university libraries. … In summary, the book is well written with some informative and detailed protocols for plant molecular biologists … .” (Stephen Byrne and Toni Wendt, Annals of Botany, Vol. 107 (4), 2011)

Bibliographic Information

  • Book Title: Plant Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: J.P. Gustafson, Peter Langridge, Daryl J. Somers

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-427-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-58829-997-0Published: 19 March 2009

  • Softcover ISBN: 978-1-61737-873-7Published: 20 January 2011

  • eBook ISBN: 978-1-59745-427-8Published: 05 April 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 362

  • Number of Illustrations: 74 b/w illustrations

  • Topics: Plant Sciences, Plant Genetics and Genomics, Gene Expression

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access