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Macrophages and Dendritic Cells

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Neil E. Reiner
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  • Provides an easily accessible reference volume for state-of-the-art experimentation by leading researchers in the field
  • Covers a wide range of approaches for the isolation and characterization of macrophages and dendritic cells
  • Provides detailed step-by-step protocols to modify gene expression in cells that are notoriously resistant to genetic manipulation
  • Provides approaches for detailed quantitative functional analyses including activation states and lipid signaling
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 531)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Generation of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors

    • Georgia Schäfer, Gordon D. Brown*
    Pages 1-15

  3. Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite Interactions

    • Alessandra Mortellaro, Matteo Urbano, Stefania Citterio, Maria Foti, Francesca Granucci, Paola Ricciardi-Castagnoli
    Pages 17-27

  4. Macrophage Activation: Classical Vs. Alternative

    • Andrea Classen, Jorge Lloberas, Antonio Celada
    Pages 29-43

  5. Analysis of Macrophage Phagocytosis: Quantitative Assays of Phagosome Formation and Maturation Using High-Throughput Fluorescence Microscopy

    • Benjamin E. Steinberg, Sergio Grinstein
    Pages 45-56

  6. Detection of Activated Rab7 GTPase with an Immobilized RILP Probe

    • Jim Sun, Ala-Eddine Deghmane, Cecilia Bucci, Zakaria Hmama
    Pages 57-69

  7. Probing In Vivo Origins of Mononuclear Phagocytes by Conditional Ablation and Reconstitution

    • Chen Varol, Limor Landsman, Steffen Jung
    Pages 71-87

  8. Multivalent Protein Probes for the Identification and Characterization of Cognate Cellular Ligands for Myeloid Cell Surface Receptors

    • Hsi-Hsien Lin, Gin-Wen Chang, Yi-Shu Huang, Cheng-Chih Hsiao, Martin Stacey, Siamon Gordon
    Pages 89-101

  9. Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose Receptor

    • Luisa Martinez-Pomares
    Pages 103-122

  10. The Expression of Exogenous Genes in Macrophages: Obstacles and Opportunities

    • Xia Zhang, Justin P. Edwards, David M. Mosser
    Pages 123-143

  11. Genetic Modification of Murine Dendritic Cells by RNA Transfection

    • Maurizio Ceppi, Enrico Schmidt, Philippe Pierre
    Pages 145-156

  12. Recording Phagosome Maturation Through the Real-Time, Spectrofluorometric Measurement of Hydrolytic Activities

    • Robin M. Yates, Albin Hermetter, David G. Russell
    Pages 157-171

  13. Derivation and Characterization of Murine Alternatively Activated (M2) Macrophages

    • Victor W.H. Ho, Laura M. Sly
    Pages 173-185

  14. Isolation and Culture of Human Hematopoietic Progenitors for Studies of Dendritic Cell Biology

    • Mattias Svensson
    Pages 187-202

  15. Generation, Culture and Flow-Cytometric Characterization of Primary Mouse Macrophages

    • Ulrike Schleicher, Christian Bogdan
    Pages 203-224

  16. Analyzing Classical and Alternative Macrophage Activation in Macrophage/Neutrophil-Specific IL-4 Receptor-Alpha-Deficient Mice

    • Frank Brombacher, Berenice Arendse, Reagon Peterson, Alexandra Hölscher, Christoph Hölscher
    Pages 225-252

  17. Analysis of Matrix Metalloproteinase Secretion by Macrophages

    • Paul T. Elkington, Justin A. Green, Jon S. Friedland
    Pages 253-265

  18. Identification of Pathogen Receptors on Dendritic Cells to Understand their Function and to Identify New Drug Targets

    • Lot de Witte, Marein A.W.P. de Jong, Jeroen den Dunnen, Yvette van Kooyk, Teunis B.H. Geijtenbeek
    Pages 267-285

  19. Stable Lentiviral Vector-Mediated Gene Silencing in Human Monocytic Cell Lines

    • Jimmy S. Lee, Neil E. Reiner
    Pages 287-300

  20. Use of Fluorescent Probes to Detect Lipid Signaling Intermediates in Macrophages

    • Vivian Kwok, Eric Vachon, Gregory P. Downey
    Pages 301-328
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Keywords

About this book

In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential. In Macrophages and Dendritic Cells: Methods and Protocols, expert researchers contribute laboratory protocols involving these two vital cell types functioning at the junction of the innate and acquired immune systems. The volume delves first into isolation and cell culturing then continues with topics such as phagocytosis, genetic manipulation, macrophage activation, and lipid signaling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Macrophages and Dendritic Cells: Methods and Protocols provides a timely and useful guide for both seasoned investigators and neophytes pursuing this imperative field of study.

Reviews

From the reviews: “This book, part of the Methods in Molecular Biology series, provides detailed descriptions of techniques that … may be adapted to individual research projects. … a book on state-of-the-art approaches to investigate the roles of macrophages and dendritic cells in health and disease to better understand their function in the host response. … useful for graduate students, postdoctoral fellows, and experienced investigators. … the protocols are described in great detail in this book, which should be helpful to anyone considering establishing a new research procedure.” (Marion C. Cohen, Doody’s Review Service, December, 2009)

Bibliographic Information

  • Book Title: Macrophages and Dendritic Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Neil E. Reiner

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-396-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-58829-972-7Published: 01 April 2009

  • Softcover ISBN: 978-1-62703-849-2Published: 26 November 2014

  • eBook ISBN: 978-1-59745-396-7Published: 05 April 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 368

  • Topics: Immunology, Cell Culture

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