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Cancer Stem Cells

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. John S. Yu
    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides easy to follow protocols in this technically challenging field
  • Presents a cohesive review of a variety of methods to isolate, identify, and study cancer stem cells
  • Is a timely contribution in this nascent field and will level the playing field of investigators of cancer stem cells
  • Presents a comprehensive, step-by-step guide by leading researchers in the field of cancer stem cells
  • Brings to light methods that have been mired in mystery
  • Brings together divergent viewpoints and methods to isolate the same population of cells, from prospective isolation using surface markers to stem cell propagation assays

Part of the book series: Methods in Molecular Biology (MIMB, volume 568)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Defining Cancer Stem Cells by Xenotransplantation in Zebrafish

    • Michael C. Dovey, Leonard I. Zon
    Pages 1-5

  3. Analysis of Cancer Stem Cell Metastasis in Xenograft Animal Models

    • Yibin Kang
    Pages 7-19

  4. Identification of Murine and Human Acute Myeloid Leukemia Stem Cells

    • Aniruddha J. Deshpande, Farid Ahmed, Christian Buske
    Pages 21-35

  5. Methods for Analysis of Brain Tumor Stem Cell and Neural Stem Cell Self-Renewal

    • Ichiro Nakano, Harley I. Kornblum
    Pages 37-56

  6. Prospective Identification of Cancer Stem Cells with the Surface Antigen CD133

    • Kyeung Min Joo, Do-Hyun Nam
    Pages 57-71

  7. Neurosphere Culture and Human Organotypic Model to Evaluate Brain Tumor Stem Cells

    • Hugo Guerrero-Cázares, Kaisorn L. Chaichana, Alfredo Quiñones-Hinojosa
    Pages 73-83

  8. Methodologies in Assaying Prostate Cancer Stem Cells

    • Hangwen Li, Ming Jiang, Sofia Honorio, Lubna Patrawala, Collene R. Jeter, Tammy Calhoun-Davis et al.
    Pages 85-138

  9. Characterization of Nonmalignant and Malignant Prostatic Stem/Progenitor Cells by Hoechst Side Population Method

    • Murielle Mimeault, Surinder K. Batra
    Pages 139-149

  10. Anchorage-Independent Growth of Prostate Cancer Stem Cells

    • Shunyou Wang
    Pages 151-160

  11. Identification of Human Pancreatic Cancer Stem Cells

    • Chenwei Li, C. J. Lee, Diane M. Simeone
    Pages 161-173

  12. Cancer Stem Cells in Head and Neck Squamous Cell Carcinoma

    • Laurie Ailles, Mark Prince
    Pages 175-193

  13. Pituitary Adenoma Stem Cells

    • Patrizia Tunici, John S. Yu
    Pages 195-201

  14. Genome-Wide DNA Methylation Profiling: The mDIP-Chip Technology

    • Yin Shen, Shaun D. Fouse, Guoping Fan
    Pages 203-216

  15. The Contribution of Niche-Derived Factors to the Regulation of Cancer Cells

    • Julie B. Sneddon
    Pages 217-232

  16. Dendritic Cell Vaccines for Cancer Stem Cells

    • Serena Pellegatta, Gaetano Finocchiaro
    Pages 233-247

  17. Maintaining and Engineering Neural Stem Cells for Delivery of Genetically Encoded Therapy to Brain Tumors

    • Jennifer Katz, Joseph Krueger, Brunhilde Felding-Habermann, Evan Y. Snyder
    Pages 249-259

  18. Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cells

    • Vera S. Donnenberg, E. Michael Meyer, Albert D. Donnenberg
    Pages 261-279

  19. Back Matter

    Pages 281-283
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Keywords

About this book

Cancer is a devastating disease that affects millions of people in the world. Our the- pies for tumors have mostly been based on classical chemotherapy and antiproliferative treatments. More recently, directed therapies against a causative oncogene have led to prominent reduction in tumor rates for some cancers. For instance, chronic myelogenous leukemia that is due to a BCR–ABL translocation can be targeted with Gleevec. Despite these advances in individual tumors, a number of patients are treated for their primary tumors and ultimately relapse. Relapsing tumors can be due to resistance to chemotherapy or to antioncogene drugs. Another hypothesis is that heterogeneity in the tumor leads to an inability of classical chemotherapy to completely eradicate all cells of the tumor. This concept of heterogeneity has led some investigators to propose a cancer stem cell model. As patients are treated with chemotherapy, most of the dividing cells are killed, but this leaves a small subset of cells that have the ability to remake the entire tumor. These are cancer stem cells. They possess the signals of self-renewal and yet can also differentiate. If one could understand more about the cells that remain after classical chemotherapy or the cells that can remake the tumor among the heterogeneous population, this information would have a huge impact on our treatment of cancer.

Reviews

From the reviews:

“This volume in the popular Methods in Molecular Biology series provides a state-of-the art compendium of research techniques and protocols for identifying and culturing rare populations of cancer stem cells. … The audience includes basic science and clinical researchers who are interested in stem cell biology as well as mechanisms of carcinogenesis and malignancy. The book also will be valued by biomedical scientists and clinicians interested in clinical oncology. … The topic is fascinating and clinically relevant.” (Bruce A. Fenderson, Doody’s Review Service, June, 2010)

Bibliographic Information

  • Book Title: Cancer Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: John S. Yu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-280-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-58829-938-3Published: 17 August 2009

  • Softcover ISBN: 978-1-61779-753-8Published: 09 August 2012

  • eBook ISBN: 978-1-59745-280-9Published: 07 July 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 284

  • Number of Illustrations: 42 b/w illustrations, 16 illustrations in colour

  • Topics: Oncology, Cancer Research, Stem Cells, Cell Culture

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