Overview
- Editors:
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Kan Wang
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Center for Plant Transformation, Plant Science Institute, Iowa State Universtiy, Ames
Department of Agronomy, Iowa State Universtiy, Ames
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Table of contents (41 protocols)
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Front Matter
Pages i-xxiii
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Root Plants
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- Owen Wally, Jayaraj Jayaraman, Zamir K. Punja
Pages 3-12
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- Weston Msikita, Uzoma Ihemere, Dimuth Siritunga, Richard T. Sayre
Pages 13-24
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- Guo-qing Song, Ken-ichi Yamaguchi
Pages 37-44
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Turf Grasses
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- Chip Longo, Colin Lickwar, Qian Hu, Kimberly Nelson-Vasilchik, David Viola, Joel Hague et al.
Pages 83-95
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Woody Species
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- Andrew E. Newhouse, Franziska Schrodt, Charles A. Maynard, William A. Powell
Pages 99-112
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- Rubén Álvarez, Mariano Toribio, Millán Cortizo, Ricardo-Javier Ordás Fernández
Pages 113-123
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- Zenn-Zong Chen, Cheng-Kuen Ho, In-Suk Ahn, Vincent L. Chiang
Pages 125-134
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- Jan Grant, Tracy Dale, Pauline Cooper
Pages 135-141
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- Richard Meilan, Caiping Ma
Pages 143-151
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- Perumal Venkatachalam, Radha Jayashree, Karumamkandathil Rekha, Sreedharannair Sushmakumari, Sankaren Sobha, Parukkuttyamma Kumari Jayasree et al.
Pages 153-164
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Tropic Plants
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Front Matter
Pages 165-165
About this book
Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.
Reviews
From the reviews of the second edition:
"This is, as the title suggests, a laboratory manual with protocols for using Agrobacterium tumefaciens to transform plant species … . the book will be a valuable addition to the laboratory bookshelf. The protocols … are clearly set out and the notes explain things … . I was also pleased that some chapters mentioned the need to consider regulatory issues regarding disposal of genetically modified materials, something that is often ignored in lab manuals." (Heather Macdonald, SGM – Society for General Microbiology, 2007)
Editors and Affiliations
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Center for Plant Transformation, Plant Science Institute, Iowa State Universtiy, Ames
Kan Wang
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Department of Agronomy, Iowa State Universtiy, Ames
Kan Wang