Overview
- Provides current methods for experimentation with Candida albicans
- Applies Immunological, Molecular, Genetic and Mircobiological approaches
- Topics include evaluation of vaccines, virulence factor determinants, biofilms, and host cell responses, strain typing and others
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 499)
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Table of contents (18 protocols)
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Front Matter
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Immunogical Methods
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Front Matter
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Virulence Measurements: In vitro, ex vivo, and in vivo
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Front Matter
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Strain Typing and Identification
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Front Matter
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Genomics and Proteomics
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Front Matter
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Keywords
About this book
Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Protocols, expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time, the work includes in-depth descriptions of disease models of candidiasis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification. Composed in the highly successful "Methods in Molecular Biology™" series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting edge, Candida Albicans: Methods and Protocols is an invaluable source of methods for investigators in the exhilarating fields of medical and molecular mycology.
Reviews
From the reviews:
"This small book is directed specifically to researchers involved in the study of yeast associated with human disease. … this book will be used by scientists and students in the field of fungal and yeast research. … This compact book … would clearly be a good investment for scientists studying or teaching about Candida species and virulence in humans. Another positive attribute is its small size, which makes it easy to carry around and fit on a lab bench." (Rebecca T. Horvat, Doody’s Review Service, June, 2009)
"Human pathogenic fungi, especially Candida albicans, are significant opportunist pathogens of immunocompromised and medically compromised patients. … This book contains 18 succinct chapters that focus on … important aspects of molecular methodology. … Each chapter contains concise protocols, together with a useful Notes section providing tips on troubleshooting. This book will be a useful resource for researchers who work in the field. It is … a worthwhile institutional purchase." (David Coleman, Microbiology Today, November, 2009)
Editors and Affiliations
Bibliographic Information
Book Title: Candida Albicans
Book Subtitle: Methods and Protocols
Editors: Ronald L. Cihlar, Richard A. Calderone
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-151-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-58829-760-0Published: 05 February 2009
Softcover ISBN: 978-1-61737-761-7Published: 19 November 2010
eBook ISBN: 978-1-60327-151-6Published: 17 January 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 206
Number of Illustrations: 28 b/w illustrations
Topics: Microbiology, Bacteriology, Proteomics