Lipid Rafts

1. Front Matter

   Pages i-xi

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2. Overview of Membrane Rafts

   • Thomas J. McIntosh

   Pages 1-7

3. Analysis of Raft Affinity of Membrane Proteins by Detergent-Insolubility

   • Deborah A. Brown

   Pages 9-20

4. Nondetergent Isolation of Rafts

   • Mehul B. Shah, Pravin B. Sehgal

   Pages 21-28

5. Detecting Ordered Domain Formation (Lipid Rafts) in Model Membranes Using Tempo

   • Omar Bakht, Erwin London

   Pages 29-40

6. Using Monomolecular Films to Characterize Lipid Lateral Interactions

   • Rhoderick E. Brown, Howard L. Brockman

   Pages 41-58

7. Electro-Formation and Fluorescence Microscopy of Giant Vesicles With Coexisting Liquid Phases

   • Sarah L. Veatch

   Pages 59-72

8. Fluorescence Correlation Spectroscopy

   • Kirsten Bacia, Petra Schwille

   Pages 73-84

9. Multiphoton Laser-Scanning Microscopy and Spatial Analysis of Dehydroergosterol Distributions on Plasma Membrane of Living Cells

   • Avery L. McIntosh, Barbara P. Atshaves, Huan Huang, Adalberto M. Gallegos, Ann B. Kier, Friedhelm Schroeder et al.

   Pages 85-105

10. NMR Detection of Lipid Domains

    • Ivan V. Polozov, Klaus Gawrisch

    Pages 107-126

11. Lateral Diffusion Coefficients of Raft Lipids From Pulsed Field Gradient NMR

    • Greger Orädd, Göran Lindblom

    Pages 127-142

12. Saturation-Recovery Electron Paramagnetic Resonance Discrimination by Oxygen Transport (DOT) Method for Characterizing Membrane Domains

    • Witold K. Subczynski, Justyna Widomska, Anna Wisniewska, Akihiro Kusumi

    Pages 143-157

13. Plasmon-Waveguide Resonance Spectroscopy Studies of Lateral Segregation in Solid-Supported Proteolipid Bilayers

    • Zdzislaw Salamon, Savitha Devanathan, Gordon Tollin

    Pages 159-178

14. Fluorescence Recovery After Photobleaching Studies of Lipid Rafts

    • Anne K. Kenworthy

    Pages 179-192

15. Single-Molecule Tracking

    • Marija Vrljic, Stefanie Y. Nishimura, W. E. Moerner

    Pages 193-219

16. X-Ray Diffraction to Determine the Thickness of Raft and Nonraft Bilayers

    • Thomas J. McIntosh

    Pages 221-230

17. Small-Angle Neutron Scattering to Detect Rafts and Lipid Domains

    • Jeremy Pencer, Thalia T. Mills, Norbert Kucerka, Mu-Ping Nieh, John Katsaras

    Pages 231-244

18. Exploring Membrane Domains Using Native Membrane Sheets and Transmission Electron Microscopy

    • Bridget S. Wilson, Janet R. Pfeiffer, Mary Ann Raymond-Stintz, Diane Lidke, Nicholas Andrews, Jun Zhang et al.

    Pages 245-261

19. Atomic Force Microscopy of Lipid Domains in Supported Model Membranes

    • Alan R. Burns

    Pages 263-282

20. Atomistic and Coarse-Grained Computer Simulations of Raft-Like Lipid Mixtures

    • Sagar A. Pandit, H. Larry Scott

    Pages 283-302

In the past several years significant attention has been given to the analysis of the properties and functions of lateral microdomains (rafts) in biological membranes. As described in the overview chapter of this book, as well as in the introductions to many of the other chapters, there are many fundamental un- swered questions concerning the composition, structure, dynamics, and even the very existence of these membrane rafts. Therefore, a variety of sophisticated techniques have been used to study intact cell membranes, as well as model s- tems composed of specific lipids and proteins thought to be in rafts. The analyses of biological membranes have provided detailed data on intact microdomains, and the complementary studies of model systems have given critical insights on the roles of specific lipid-lipid and lipid-protein interactions in raft formation and function. The chapters in this book provide detailed information on modern methods that are currently being employed to study lipid rafts.

• Lipid
• X-ray
• electron microscopy
• fluorescence
• magnetic resonance
• microscopy
• molecular biology
• proteins
• spectroscopy
• Biological Microscopy

• Department of Cell Biology, Duke University Medical Center, Durham

  Thomas J. McIntosh

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