Overview
- Shows a diverse set of instructive examples from probe design to applications in clinical settings guided by experts in the field who provide easy to follow experiments
- The book commences with an introduction to the basic principles of fluorescence
- Extends the potential of realtime PCR
- Contributions advocate simplifications of the probe technologies
- Presents a new simplified molecular beacon design EasyBeacons and demonstrates the utility in DNA methylation profiling
Part of the book series: Methods in Molecular Biology (MIMB, volume 429)
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Table of contents (18 protocols)
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Front Matter
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Introduction
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Front Matter
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Applications
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Front Matter
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Non-Nucleic Acid Targets
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Front Matter
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Keywords
About this book
Significant DNA sequence data has been made available by several important genome projects. The allocation of functions to a mere string of the four letters in the code of life plays a major if not predominant role of ongoing and future research. Guided by experts in the field, Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols, provides easy-to-follow experiments, commences with an introduction to the basic principles of fluorescence, and shows a diverse set of instructive examples, from probe design to applications in clinical settings, molecular beacon technology, the identification of transgenic mice, along with the potential of realtime PCR. While originally being used for fluorogenic nucleic acid probes with a stem-loop structure, molecular beacon now refers to a whole family of fluorescence-up probes. Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols summarizes all the latest findings and explores powerful techniques designed for geneticist, biotechnologists, molecular and cellular biologists, researchers and developers.
Editors and Affiliations
Bibliographic Information
Book Title: Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols
Editors: Andreas Marx, Oliver Seitz
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-040-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-700-6Published: 03 April 2008
Softcover ISBN: 978-1-61737-726-6Published: 10 December 2010
eBook ISBN: 978-1-60327-040-3Published: 11 August 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 266
Number of Illustrations: 60 b/w illustrations, 17 illustrations in colour
Topics: Internal Medicine, Human Genetics, Biotechnology, Animal Genetics and Genomics