Overview
- Detailed description of quality control testing of vectors
- Methods for determination of virus titers
- Sensitive assays for testing viral DNA presence in samples
- Developing new, permissive immunocompetent animal models
- Characterization of specific Ad proteins (hexon, fiber, or protease/proteinase)
- Procedures for microinjection, transformation assays, and NK cell-mediated cytolysis
- Analysis of phylogenetics and genetic variability
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Medicine (MIMM, volume 130)
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Table of contents (18 protocols)
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Front Matter
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Back Matter
Keywords
About this book
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise.
Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.
Reviews
"...provdes some good advice on using and growing adenovirus vectors." -Doody's Book Review, Weighted Numerical Score: 62 - 2 Stars
Reviews of the First edition:
"This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them. . .a very comprehensive methods book that will be a laboratory standard for years to come."-Microbiology Today
". . .this book constitutes an excellent reference text . . .this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses."-FEBS Letters
Editors and Affiliations
Bibliographic Information
Book Title: Adenovirus Methods and Protocols
Book Subtitle: Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models
Editors: William S. M. Wold, Ann E. Tollefson
Series Title: Methods in Molecular Medicine
DOI: https://doi.org/10.1385/1597451665
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-598-9Published: 27 February 2007
Softcover ISBN: 978-1-61737-669-6Published: 10 November 2010
eBook ISBN: 978-1-59745-166-6Published: 02 February 2008
Series ISSN: 1543-1894
Series E-ISSN: 1940-6037
Edition Number: 2
Number of Pages: XIV, 242
Number of Illustrations: 30 b/w illustrations
Topics: Virology, Cell Biology, Human Genetics, Oncology