Glycovirology Protocols

1. Front Matter

   Pages i-x

   PDF

2. Viruses and Glycosylation

   • Richard J. Sugrue

   Pages 1-13

3. Interaction Between Respiratory Syncytial Virus and Glycosaminoglycans, Including Heparan Sulfate

   • Louay K. Hallak, Steven A. Kwilas, Mark E. Peeples

   Pages 15-34

4. Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System

   • Sehaam Khan, Mah-Lee Ng, Yee-Joo Tan

   Pages 35-50

5. The C Type Lectins DC-SIGN and L-SIGN

   • Pierre-Yves Lozach, Laura Burleigh, Isabelle Staropoli, Ali Amara

   Pages 51-68

6. Functional Analysis of the N-Linked Glycans Within the Fusion Protein of Respiratory Syncytial Virus

   • Ping Li, Helen W. McL. Rixon, Gaie Brown, Richard J. Sugrue

   Pages 69-83

7. Expression and Purification of Viral Glycoproteins Using Recombinant Vaccinia Viruses for Functional and Structural Studies

   • Zhu-Nan Li, David A. Steinhauer

   Pages 85-95

8. The Use of Two-Dimensional SDS-PAGE to Analyze the Glycan Heterogeneity of the Respiratory Syncytial Virus Fusion Protein

   • Terence P. McDonald, Richard J. Sugrue

   Pages 97-108

9. The Use of Monoclonal Antibodies and Lectins to Identify Changes in Viral Glycoproteins That are Influenced by Glycosylation

   • Joanna Rawling, José A. Melero

   Pages 109-125

10. Expression, Glycosylation, and Modification of the Spike (S) Glycoprotein of SARS CoV

    • Shuo Shen, Timothy H. P. Tan, Yee-Joo Tan

    Pages 127-135

11. Analysis of Glycoproteins of Viruses in the Family Bunyaviridae

    • Xiaohong Shi, Richard M. Elliott

    Pages 137-148

12. Secretion of the Respiratory Syncytial Virus Fusion Protein From Insect Cells Using the Baculovirus Expression System

    • Boon-Huan Tan, Gaie Brown, Richard J. Sugrue

    Pages 149-161

13. Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris

    • Boon-Huan Tan, Jian Lin Fu, Richard J. Sugrue

    Pages 163-176

14. Cloning, Expression, and Functional Analysis of Patient-Derived Hepatitis C Virus Glycoproteins

    • Alexander W. Tarr, Ania M. Owsianka, Alexandra Szwejk, Jonathan K. Ball, Arvind H. Patel

    Pages 177-197

15. Back Matter

    Pages 199-205

    PDF

Glycovirology Protocols reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins.

• Hepatitis
• Polyacrylamid-Gelelektrophorese
• antibody
• biology
• infection
• protein
• proteins
• virology
• virus
• infectious diseases

• MRC Virology Unit, Institute of Virology, Glasgow, UK

  Richard J. Sugrue

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