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Peptide Characterization and Application Protocols

  • Book
  • © 2007

Overview

Editors:
  1. Gregg B. Fields

    1. Department of Chemistry and Biochemistry, Florida Atlantic University, Boca Raton

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  • HPLC analysis and purification of peptides
  • MS/MS spectra identification of proteins
  • Peptide-mediated targeting of liposomes to tumor cells

Part of the book series: Methods in Molecular Biology (MIMB, volume 386)

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Table of contents (13 protocols)

  1. Front Matter

    Pages i-xi
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  2. Characterization

    1. Front Matter

      Pages 1-1
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    2. HPLC Analysis and Purification of Peptides

      • Colin T. Mant, Yuxin Chen, Zhe Yan, Traian V. Popa, James M. Kovacs, Janine B. Mills et al.
      Pages 3-55

    3. Identification of Proteins Based on MS/MS Spectra and Location of Posttranslational Modifications

      • Kathryn L. Stone, Myron Crawford, Walter McMurray, Nancy Williams, Kenneth R. Williams
      Pages 57-77

    4. Determination of Phosphorylated and O-Glycosylated Sites by Chemical Targeting (CTID) at Ambient Temperature

      • Gary M. Hathaway
      Pages 79-93

    5. Synthesis, Biosynthesis, and Characterization of Transmembrane Domains of a G Protein—Coupled Receptor

      • Fred Naider
      Pages 95-121

  3. Applications

    1. Front Matter

      Pages 122-122
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    2. Application of Topologically Constrained Mini-Proteins as Ligands, Substrates, and Inhibitors

      • Janelle L. Lauer-Fields, Dmitriy Minond, Keith Brew, Gregg B. Fields
      Pages 125-166

    3. Proteolytic Profiling of the Extracellular Matrix Degradome

      • Diane Baronas-Lowell, Janelle L. Lauer-Fields, Mohammad Al-Ghoul, Gregg B. Fields
      Pages 167-202

    4. β-Amyloid Protein Aggregation

      • Marcus A. Etienne, Nadia J. Edwin, Jed P. Aucoin, Paul S. Russo, Robin L. McCarley, Robert P. Hammer
      Pages 203-225

    5. Radiometal-Labeled Somatostatin Analogs for Applications in Cancer Imaging and Therapy

      • Jason S. Lewis, Carolyn J. Anderson
      Pages 227-240

    6. Cell-Penetrating Proline-Rich Peptidomimetics

      • Josep Farrera-Sinfreu, Ernest Giralt, Miriam Royo, Fernando Albericio
      Pages 241-267

    7. Peptide-Mediated Targeting of Liposomes to Tumor Cells

      • Evonne M. Rezler, David R. Khan, Raymond Tu, Matthew Tirrell, Gregg B. Fields
      Pages 269-298

    8. Peptide-Mediated Delivery of Nucleic Acids into Mammalian Cells

      • Sébastien Deshayes, Federica Simeoni, May C. Morris, Gilles Divita, Frédéric Heitz
      Pages 299-308

    9. Broth Microdilution Antibacterial Assay of Peptides

      • Laszlo Otvos, Mare Cudic
      Pages 309-320

    10. Depsipeptide Synthesis

      • Maciej Stawikowski, Predrag Cudic
      Pages 321-339

  4. Back Matter

    Pages 341-342
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Keywords

About this book

Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including high-performance liquid chromatography (HPLC) for purification and evaluation of peptides and mass spectrometry (MS) for the analysis of standard and modified peptides. Innovative methods of synthesis and characterization of membrane peptides are also presented. Part II, Application, presents a variety of specific applications for synthetic peptides. The collected protocols demonstrate the systematic manner in which peptides can be constructed, analyzed, and applied to attack contemporary problems in biochemistry, and this book will be an indispensable aid in the pursuit of new directions in peptide research.

Editors and Affiliations

  • Department of Chemistry and Biochemistry, Florida Atlantic University, Boca Raton

    Gregg B. Fields

Bibliographic Information

  • Book Title: Peptide Characterization and Application Protocols

  • Editors: Gregg B. Fields

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-430-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-550-7Published: 11 May 2007

  • Softcover ISBN: 978-1-61737-637-5Published: 19 November 2010

  • eBook ISBN: 978-1-59745-430-8Published: 01 April 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 342

  • Number of Illustrations: 98 b/w illustrations, 4 illustrations in colour

  • Topics: Biotechnology, Cell Biology, Biochemistry, general

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