PRINS and In Situ PCR Protocols

1. Front Matter

   Pages i-xvi

   PDF

2. Basic Methodology

   1. Front Matter

      Pages 1-1

      PDF

   2. New Rapid Multicolor PRINS Protocol

      • Ju Yan, Macoura Gadji, Kada Krabchi, Régen Drouin

      Pages 3-13

   3. PRINS Combined With Peptide Nucleic Acid Labeling

      • Franck Pellestor, Petra Paulasova

      Pages 15-22

   4. PRINS Combined With Indirect Immunofluorescence

      • Philippe Coullin

      Pages 23-32

   5. PRINS for the Detection of Unique Sequences

      • Stephen S. Wachtel, Avirachan T. Tharapel

      Pages 33-40

   6. Single-Copy and Point Mutation Analysis (Rolling-Circle PRINS)

      • Jørn Koch

      Pages 41-48

   7. Analysis of Sperm Aneuploidy by PRINS

      • Franck Pellestor, Brigitte Andréo, Jacques Puechberty, Geneviève Lefort, Pierre Sarda

      Pages 49-59

   8. In Situ PCR Protocols

      • Omar Bagasra, Twaina Harris

      Pages 61-78

3. Research and Clinical Applications

   1. Front Matter

      Pages 79-79

      PDF

   2. Detection and Sizing of Telomeric and Other Simple Repeats by Dideoxy-PRINS

      • Jørn Koch

      Pages 81-88

   3. PRINS Evaluation of Chromosome Instability in Mammalian Cells by Detection of Repetitive DNA Sequences in Micronuclei

      • Antonella Russo

      Pages 89-104

   4. PRINS for the Detection of Gene Deletions in Cancer

      • Avirachan T. Tharapel, Stephen S. Wachtel

      Pages 105-113

   5. Combined Fluorescence In Situ Hybridization and PRINS for the Analysis of the Dystrophin Gene

      • Liborio Stuppia, Dario La Sala, Caterina Cinti

      Pages 115-122

   6. Brain Tissue Preparations for Chromosomal PRINS Labeling

      • Ivan Y. Iourov, Svetlana G. Vorsanova, Franck Pellestor, Yuri B. Yurov

      Pages 123-132

   7. PRINS on Plant Chromosomes

      • Jirí Macas, Alice Navrátilová, Marie Kubaláková, Jaroslav Dolezel

      Pages 133-139

   8. Dual-Color PRINS for In Situ Detection of Fetal Cells in Maternal Blood

      • Kada Krabchi, Macoura Gadji, Ju Yan, Régen Drouin

      Pages 141-149

   9. PRINS as an Efficient Tool for Aneuploidy Assessment in Human Oocytes and Preimplantation Embryos

      • Franck Pellestor, Brigitte Andréo, Tal Anahory, Samir Hamamah

      Pages 151-160

   10. Microdissection With PCR In Situ

       • Allen T. Christian, Christine Hara

       Pages 161-168

   11. Application of Reverse Transcription In Situ PCR in Cancer Analysis

       • Tamotsu Takeuchi, Yoshihiro Adachi, Hiroshi Sonobe, Yuji Ohtsuki

       Pages 169-179

The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.

• DNA
• Mutation
• PCR
• chromosome
• genes
• hybridization
• transcription

From Reviews of the First Edition...

"...essential for any laboratory contemplating exploiting this powerful technique." -Microbiology Today

"...[a] splendid little book which covers the field quite well,,," -FEBS Letters

"...accessible and very practical...recommend[ed]...to any laboratory with an operational PCR machine...a valuable addition to the methods literature." -Quarterly Review of Biology

"...well written...contains sufficient detail for a novice to successfully complete the experiment...recommend[ed]...for libraries as well as individual researchers interested in applying PRINS technology." -Doody's Health Sciences Book Review Journal

• Institute of Human Genetics, Montpellier, France

  Franck Pellestor

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