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New and Emerging Proteomic Techniques

  • Book
  • © 2006

Overview

Authors:
  1. Dobrin Nedelkov

    1. Intrinsic Bioprobes Inc., Tempe

    View author publications

    You can also search for this author in PubMed   Google Scholar

  2. Randall W. Nelson

    1. Intrinsic Bioprobes Inc., Tempe

    View author publications

    You can also search for this author in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 328)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. On-Chip Protein Synthesis for Making Microarrays

    • Niroshan Ramachandran, Eugenie Hainsworth, Gokhan Demirkan, Joshua LaBaer
    Pages 1-14

  3. RCA-Enhanced Protein Detection Arrays

    • Brian B. Haab, Paul M. Lizardi
    Pages 15-29

  4. Antibody Microarrays Using Resonance Light-Scattering Particles for Detection

    • Bernhard H. Geierstanger, Petri Saviranta, Achim Brinker
    Pages 31-50

  5. Chemical Proteomics Profiling of Proteasome Activity

    • Martijn Verdoes, Celia R. Berkers, Bogdan I. Florea, Paul F. van Swieten, Herman S. Overkleeft, Huib Ovaa
    Pages 51-69

  6. Two-Dimensional Difference Gel Electrophoresis

    • Terence L. Wu
    Pages 71-95

  7. Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors

    • Ewa Folta-Stogniew
    Pages 97-112

  8. Surface Plasmon Resonance Imaging Measurements of Protein Interactions With Biopolymer Microarrays

    • Terry T. Goodrich, Alastair W. Wark, Robert M. Corn, Hye Jin Lee
    Pages 113-130

  9. Surface Plasmon Resonance Mass Spectrometry for Protein Analysis

    • Dobrin Nedelkov, Randall W. Nelson
    Pages 131-139

  10. High-Throughput Affinity Mass Spectrometry

    • Urban A. Kiernan, Dobrin Nedelkov, Eric E. Niederkofler, Kemmons A. Tubbs, Randall W. Nelson
    Pages 141-150

  11. Isotope-Coded Affinity Tags for Protein Quantification

    • Christopher M. Colangelo, Kenneth R. Williams
    Pages 151-158

  12. Proteomic Analysis by Multidimensional Protein Identification Technology

    • Laurence Florens, Michael P. Washburn
    Pages 159-175

  13. Isolation of Glycoproteins and Identification of Their N-Linked Glycosylation Sites

    • Hui Zhang, Ruedi Aebersold
    Pages 177-185

  14. N-Glycosylation Analysis Using the StrOligo Algorithm

    • Martin Ethier, Daniel Figeys, Hélène Perreault
    Pages 187-197

  15. MALDI-MS Data Analysis for Disease Biomarker Discovery

    • Weichuan Yu, Baolin Wu, Junfeng Liu, Xiaoye Li, Kathy Stone, Kenneth R. Williams et al.
    Pages 199-216

  16. Using the Global Proteome Machine for Protein Identification

    • Ronald C. Beavis
    Pages 217-228

  17. Back Matter

    Pages 229-232
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Keywords

About this book

Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Reviews

From the reviews:

"It is no surprise that a book of this title is somewhat eclectic. … Methodologies are well written and useful, emphasizing that proteins and function can be studied at differing levels of resolution. … this book will be useful for beginners, though it is really more for the initiated seeking inspiration." (Dr. Mike J. Naldrett, Microbiology Today, November, 2006)

“New and Emerging Proteomics Techniques contains detailed descriptions of modern proteomics techniques written by experts in the field. … Overall, the book’s background information and detailed protocols and notes would be of interest to researchers in many areas who are interested in knowing more about the types of studies now made possible by these powerful, combined or individual proteomics techniques, and how they might be applied to current questions in basic and medical science.”­­­ (Constance J. Jeffery, Journal of the American Society for Mass Spectrometry, Vol. 18, 2007)

Authors and Affiliations

  • Intrinsic Bioprobes Inc., Tempe

    Dobrin Nedelkov, Randall W. Nelson

Bibliographic Information

  • Book Title: New and Emerging Proteomic Techniques

  • Authors: Dobrin Nedelkov, Randall W. Nelson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/159745026X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-519-4Published: 01 March 2006

  • Softcover ISBN: 978-1-61737-615-3Published: 16 December 2010

  • eBook ISBN: 978-1-59745-026-3Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: 248

  • Number of Illustrations: 67 b/w illustrations

  • Topics: Biochemistry, general

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