Skip to main content
SpringerLink
Log in
Book cover
  • Book
  • © 2006

Microfluidic Techniques

Reviews and Protocols

Editors:

  1. Shelley D. Minteer

    1. Department of Chemistry, Saint Louis University, St. Louis

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 321)

Sections

Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    PDF

  2. Microfabrication Methods

    1. Front Matter

      Pages 3-3
      PDF

    2. Overview of Advances in Microfluidics and Microfabrication

      • Shelley D. Minteer, Christine M. Moore
      Pages 1-2

    3. Introduction to Microfabrication Techniques

      • Rabih Zaouk, Benjamin Y. Park, Marc J. Madou
      Pages 5-15

    4. Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds

      • Rabih Zaouk, Benjamin Y. Park, Marc J. Madou
      Pages 17-21

    5. Fabrication of Microelectrodes Using the Lift-Off Technique

      • Benjamin Y. Park, Rabih Zaouk, Marc J. Madou
      Pages 23-26

  3. Biological Applications

    1. Front Matter

      Pages 44-44
      PDF

    4. Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays

      • Gloria Thomas, Emad M. El-Giar, Laurie E. Locascio, Michael J. Tarlov
      Pages 83-95

    5. Polymerase Chain Reaction in Miniaturized Systems:

      • Kalee D. Spitzack, Victor M. Ugaz
      Pages 97-129

    6. Polymerase Chain Reaction on Microchips

      • Maria C. Carles, Nikolaus J. Sucher
      Pages 131-140

    7. A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes

      • Xiaojun Zhao, David T. Pierce, Yanfu Huan
      Pages 141-155

    8. Microfluidic Ethanol Biobatteries on a Microchip

      • Shelley D. Minteer, Christine M. Moore
      Pages 157-166

    9. Microfluidic Chambers for Cell Migration and Neuroscience Research

      • Anne M. Taylor, Seog Woo Rhee, Noo Li Jeon
      Pages 167-177

    12. Microscale Integrated Sperm Sorter

      • Yaokuang Chung, Xiaoyue Zhu, Wei Gu, Gary D. Smith, Shuichi Takayama
      Pages 227-244
Back to top

About this book

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Back to top

Keywords

Back to top

Editors and Affiliations

  • Department of Chemistry, Saint Louis University, St. Louis

    Shelley D. Minteer

Back to top

Bibliographic Information

  • Book Title: Microfluidic Techniques

  • Book Subtitle: Reviews and Protocols

  • Editors: Shelley D. Minteer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599974

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-517-0Published: 15 November 2005

  • Softcover ISBN: 978-1-61737-613-9Published: 09 December 2010

  • eBook ISBN: 978-1-59259-997-4Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 248

  • Topics: Cell Biology

Back to top

Publish with us

Policies and ethics

Back to top
Access via your institution

Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Search

Navigation