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DNA Repair Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Daryl S. Henderson

    1. Pharmacological Sciences, SUNY Stony Brook, Stony Brook

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 314)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating

    • Malgorzata Z. Zdzienicka
    Pages 1-7

  3. Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes

    • Mathilde Fréchet, Valérie Bergoglio, Odile Chevallier-Lagente, Alain Sarasin, Thierry Magnaldo
    Pages 9-23

  4. Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities

    • William W. Au, Salama A. Salama
    Pages 25-42

  5. Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation

    • Shruti Nagar, James J. Corcoran, William F. Morgan
    Pages 43-50

  6. Inhibition of DNA Synthesis by Ionizing Radiation

    • Nicolaas G. J. Jaspers, Malgorzata Z. Zdzienicka
    Pages 51-59

  7. Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication

    • George Iliakis, Ya Wang, Hong Yan Wang
    Pages 61-71

  8. Cytometric Assessment of Histone H2AX Phosphorylation

    • Xuan Huang, Zbigniew Darzynkiewicz
    Pages 73-80

  9. Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Cell Proliferation (DNA Replication)

    • Zbigniew Darzynkiewicz, Xuan Huang, Masaki Okafuji
    Pages 81-93

  10. In Vitro Rejoining of Double-Strand Breaks in Genomic DNA

    • George Iliakis, Nge Cheong
    Pages 95-108

  11. Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR

    • Michael J. Villalobos, Christopher J. Betti, Andrew T. M. Vaughan
    Pages 109-121

  12. Plasmid-Based Assays for DNA End-Joining In Vitro

    • George Iliakis, Bustanur Rosidi, Minli Wang, Huichen Wang
    Pages 123-131

  13. Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants

    • Rodney S. Nairn, Gerald M. Adair
    Pages 133-154

  14. Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage

    • R. Michael Anson, Penelope A. Mason, Vilhelm A. Bohr
    Pages 155-181

  15. Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells

    • Janine H. Santos, Joel N. Meyer, Bhaskar S. Mandavilli, Bennett Van Houten
    Pages 183-199

  16. Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR

    • Gerd P. Pfeifer
    Pages 201-214

  17. Immunochemical Detection of UV-Induced DNA Damage and Repair

    • Marcus S. Cooke, Alistair Robson
    Pages 215-228

  18. A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts

    • Shirley McCready
    Pages 229-238

  19. Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay

    • David L. Mitchell
    Pages 239-249

  20. DNA Damage Quantitation by Alkaline Gel Electrophoresis

    • Betsy M. Sutherland, Paula V. Bennett, John C. Sutherland
    Pages 251-273
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Keywords

About this book

The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new subtitle, Mammalian Systems. There are two reasons for this fresh emphasis, both of them pragmatic: to cater to the interests of what is now a largely mammalocentric DNA repair field, and to expedite editing and prod- tion of this volume. Although DNA Repair Protocols: Mammalian Systems is a smaller book than its predecessor, it actually contains a greater variety of methods. Fourteen of the book’s thirty-two chapters are entirely new and areas of redundancy present in the first edition have been eliminated here (for example, now just two chapters describe assays for nucleotide excision repair [NER], rather than seven). All eighteen returning chapters have been revised, many of them ext- sively. In order to maintain a coherent arrangement of topics, the four-part p- titioning seen in the first edition was dispensed with and chapters concerned with ionizing radiation damage and DNA strand breakage and repair were re- cated to near the front of the book. Finally, an abstract now heads each chapter.

Reviews

"...It will serve as an essential reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches." - Anticancer Research

"...must for any science library supporting a molecular biology program." - E-Streams

"An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher." - Lavoisier Librairie

"...an extremely useful resource for any laboratory with an interest in the vibrant field of DNA repair.  Any good laboratory manueal should be not only a useful reference guide but also a source of inspiration, and it is undoubtedly true that DNA Repair Protocols: Mammalian Systems succeeds on both counts." -Int. J. Radiation Oncology Biol. Phys.

"This is the definite book on the subject not only for those working in the field of DNA repair but also for those interested in genetic toxicology and chemical safety." -BTS Newsletter

Editors and Affiliations

  • Pharmacological Sciences, SUNY Stony Brook, Stony Brook

    Daryl S. Henderson

Bibliographic Information

  • Book Title: DNA Repair Protocols

  • Editors: Daryl S. Henderson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599737

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-513-2Published: 01 October 2005

  • Softcover ISBN: 978-1-61737-611-5Published: 19 November 2010

  • eBook ISBN: 978-1-59259-973-8Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 498

  • Topics: Biochemistry, general, Cell Biology

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