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Fibrosis Research

Methods and Protocols

  • Book
  • © 2005

Overview

Editors:
  1. John Varga

    1. Feinberg School of Medicine, Northwestern University, Chicago

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  2. David A. Brenner

    1. College of Physicians and Surgeons, Columbia University, New York

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    You can also search for this editor in PubMed   Google Scholar

  3. Sem H. Phan

    1. University of Michigan Medical School, Ann Arbor

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Part of the book series: Methods in Molecular Medicine (MIMM, volume 117)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. The Clinical Scope of Fibrosis

    1. Front Matter

      Pages 1-1
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    2. Pulmonary Fibrosis

      • David A. Zisman, Michael P. Keane, John A. Belperio, Robert M. Strieter, Joseph P. Lynch III
      Pages 3-44

    3. Renal Fibrosis

      • H. William Schnaper
      Pages 45-68

    4. Transforming Growth Factor-β

      • Alain Mauviel
      Pages 69-80

  3. Regulation of Extracellular Matrix Metabolism: Cultured Cells

    1. Front Matter

      Pages 81-81
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    2. Isolation and Culture of Skin Fibroblasts

      • Laure Rittié, Gary J. Fisher
      Pages 83-98

    3. Isolation and Culture of Hepatic Stellate Cells

      • Ralf Weiskirchen, Axel M. Gressner
      Pages 99-113

    4. Isolation and Phenotypic Characterization of Lung Fibroblasts

      • Carolyn J. Baglole, Sireesha Y. Reddy, Stephen J. Pollock, Steven E. Feldon, Patricia J. Sime, Terry J. Smith et al.
      Pages 115-127

    5. Methods for Measuring Type I Collagen Synthesis In Vitro

      • David C. Rishikof, Ping-Ping Kuang, Mangalalaxmy Subramanian, Ronald H. Goldstein
      Pages 129-140

    6. Methods for Assessing the Molecular Mechanisms Controlling Gene Regulation

      • Richard A. Rippe, Branko Stefanovic
      Pages 141-160

    7. Methods for Measuring TGF-β Using Antibodies, Cells, and Mice

      • Vladimir Jurukovski, Branka Dabovic, Vesna Todorovic, Yan Chen, Daniel B. Rifkin
      Pages 161-175

  4. Studying Fibrosis Using Animal Models

    1. Front Matter

      Pages 177-177
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    2. Morphological Methods for Assessment of Fibrosis

      • Rakesh K. Kumar
      Pages 179-188

    3. Methods for Measuring Hydroxyproline and Estimating In Vivo Rates of Collagen Synthesis and Degradation

      • Robin J.
      Pages 189-207

    4. Approaches to Evaluation of Fibrogenic Pathways in Surgical Lung Biopsy Specimens

      • Cory M. Hogaboam, Kristin J. Carpenter, Holly Evanoff, Steven L. Kunkel
      Pages 209-221

    5. Animal Models for Adult Dermal Wound Healing

      • Mary Birch, Annette Tomlinson, Mark W. J. Ferguson
      Pages 223-235

    6. Modeling Liver Fibrosis in Rodents

      • Christothea Constandinou, Neil Henderson, John P. Iredale
      Pages 237-250

    7. Animal Models of Pulmonary Fibrosis

      • Mehrnaz Gharaee-Kermani, Matthew Ullenbruch, Sem H. Phan
      Pages 251-259

    8. Animal Models of Renal Fibrosis

      • Michael Zeisberg, Mary A. Soubasakos, Raghu Kalluri
      Pages 261-272
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Keywords

About this book

Leading investigators review the highlights of current fibrosis research and the experimental methodologies used uncover the mechanisms that drive it. In their discussion of research methodologies utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. The protocols follow the successful Methods in Molecular Medicineâ„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Reviews

"...a must for the shelf of every researcher and physician interested in fibrotic diseases." -Rheumatology

Editors and Affiliations

  • Feinberg School of Medicine, Northwestern University, Chicago

    John Varga

  • College of Physicians and Surgeons, Columbia University, New York

    David A. Brenner

  • University of Michigan Medical School, Ann Arbor

    Sem H. Phan

Bibliographic Information

  • Book Title: Fibrosis Research

  • Book Subtitle: Methods and Protocols

  • Editors: John Varga, David A. Brenner, Sem H. Phan

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592599400

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-479-1Published: 08 August 2005

  • Softcover ISBN: 978-1-61737-592-7Published: 10 November 2010

  • eBook ISBN: 978-1-59259-940-0Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XVI, 392

  • Number of Illustrations: 80 b/w illustrations

  • Topics: Pneumology/Respiratory System

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