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Handbook of ELISPOT

Methods and Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Alexander E. Kalyuzhny

    1. R&D Systems Inc.,, Minneapolis

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Part of the book series: Methods in Molecular Biology (MIMB, volume 302)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Elispot Assay Setup and Data Analysis

    1. Front Matter

      Pages 1-1
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    2. ELISPOT Assay

      • Jonathon D. Sedgwick
      Pages 3-14

    3. Chemistry and Biology of the ELISPOT Assay

      • Alexander E. Kalyuzhny
      Pages 15-31

    4. Membranes and Membrane Plates Used in ELISPOT

      • Alan J. Weiss
      Pages 33-49

    5. Standardization and Validation Issues of the ELISPOT Assay

      • Sylvia Janetzki, Josephine H. Cox, Neal Oden, Guido Ferrari
      Pages 51-86

    6. A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay

      • Angela Grant, Sarah Palzer, Chris Hartnett, Tanya Bailey, Monica Tsang, Alexander E. Kalyuzhny
      Pages 87-94

    7. Isolation of Subsets of Immune Cells

      • Carrie E. Peters, Steven M. Woodside, Allen C. Eaves
      Pages 95-115

    8. Image Analysis and Data Management of ELISPOT Assay Results

      • Paul Viktor Lehmann
      Pages 117-131

    9. High Resolution as a Key Feature to Perform Accurate ELISPOT Measurements Using Zeiss KS ELISPOT Readers

      • Wolf Malkusch
      Pages 133-150

  3. Elispot Assay in Animal Models

    1. Front Matter

      Pages 151-151
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    2. Improving the Sensitivity of the ELISPOT Analyses of Antigen-Specific Cellular Immune Responses in Rhesus Macaques

      • K. Jagannadha Sastry, Pramod N. Nehete, Bharti Nehete
      Pages 153-165

    3. Feline Cytokine ELISPOT

      • Sushila K. Nordone, Rosemary Stevens, Alora S. LaVoy, Gregg A. Dean
      Pages 167-177

    4. Measuring T-Cell Function in Animal Models of Tuberculosis by ELISPOT

      • Vanja Lazarevic, Santosh Pawar, JoAnne Flynn
      Pages 179-189

    5. Interferon-γ ELISPOT Assay for the Quantitative Measurement of Antigen-Specific Murine CD8+ T-Cells

      • Geoffrey A. Cole
      Pages 191-203

  4. Elispot Assays in Human Studies

    1. Front Matter

      Pages 205-205
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    2. Detection of Measles Virus-Specific Interferon-γ-Secreting T-Cells by ELISPOT

      • Jenna E. Ryan, Inna G. Ovsyannikova, Gregory A. Poland
      Pages 207-217

    3. Epitope Mapping in Multiple Sclerosis Using the ELISPOT Assay

      • Clara M. Pelfrey, Ioana R. Moldovan
      Pages 219-235

    4. Use of Interferon-γ ELISPOT in Monitoring Immune Responses in Humans

      • Mark Matijevic, Robert G. Urban
      Pages 237-251

    5. ELISPOT Determination of Interferon-γ T-Cell Frequencies in Patients With Autoimmune Sensorineural Hearing Loss

      • C. Arturo Solares, Vincent K. Tuohy
      Pages 253-260
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Keywords

About this book

In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.

Reviews

From the reviews:

"The book is divided into four sections, with a total of 20 chapters featuring step-by-step protocols that will enable both novice and experienced researchers to perform ELISPOT. Each chapter begins with a general Introduction, followed by a detailed Materials section and an easy-to-follow Methods section … . All the chapters are equally interesting and well presented … . a must for a cytochemist. This book will serve as a convenient reference manual for beginners and for experienced ELISPOT users." (Carlo Alberto Redi, European Journal of Histochemistry, December, 2005)

Editors and Affiliations

  • R&D Systems Inc.,, Minneapolis

    Alexander E. Kalyuzhny

Bibliographic Information

  • Book Title: Handbook of ELISPOT

  • Book Subtitle: Methods and Protocols

  • Editors: Alexander E. Kalyuzhny

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599036

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Softcover ISBN: 978-1-61737-588-0Published: 19 November 2010

  • eBook ISBN: 978-1-59259-903-5Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 322

  • Number of Illustrations: 113 b/w illustrations, 10 illustrations in colour

  • Topics: Immunology

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