Skip to main content
SpringerLink
Log in

Yeast Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Wei Xiao

    1. Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 313)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (27 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Basic Investigations in Saccharomyces cerevisiae

    • Brendan P. G. Curran, Virginia Bugeja
    Pages 1-13

  3. Isolation of Nucleic Acids

    • Michelle Hanna, Wei Xiao
    Pages 15-20

  4. Purification of Yeast Peroxisomes

    • Ben Distel, Astrid Kragt
    Pages 21-26

  5. Isolation of Yeast Plasma Membranes

    • Barry Panaretou, Peter Piper
    Pages 27-32

  6. Isolation of Yeast Mitochondria

    • Chris Meisinger, Nikolaus Pfanner, Kaye N. Truscott
    Pages 33-39

  7. Extraction of Yeast Lipids

    • Roger Schneiter, Günther Daum
    Pages 41-45

  8. Two-Dimensional Gel Electrophoresis of Total Yeast Proteins

    • Hélian Boucherie, Christelle Monribot-Espagne
    Pages 47-64

  9. Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes

    • Laura Maringele, David Lydall
    Pages 65-73

  10. Analysis of Yeast Lipids

    • Roger Schneiter, Günther Daum
    Pages 75-84

  11. Yeast Fluorescence Microscopy

    • Jiří HaÅ¡ek
    Pages 85-96

  12. Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe

    • Michelle A. Alting-Mees, Eddy P. Risseeuw, Enwu Liu, Michel Desautels, William A. Crosby, Sean M. Hemmingsen
    Pages 97-105

  13. Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method

    • R. Daniel Gietz, Robin A. Woods
    Pages 107-120

  14. Mutagenesis

    • Leslie Barbour, Michelle Hanna, Wei Xiao
    Pages 121-127

  15. Gene Disruption in the Budding Yeast Saccharomyces cerevisiae

    • Johannes H. Hegemann, Ulrich Güldener, Gabriele J. Köhler
    Pages 129-144

  16. Inducible Degron and Its Application to Creating Conditional Mutants

    • R. Jürgen Dohmen
    Pages 145-159

  17. Synthetic Lethal Screen

    • Leslie Barbour, Wei Xiao
    Pages 161-169

  18. Synthetic Genetic Array Analysis in Saccharomyces cerevisiae

    • Amy Hin Yan Tong, Charles Boone
    Pages 171-191

  19. Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates

    • Alain T. Dandjinou, Michel Larrivée, Ralf E. Wellinger, Raymund J. Wellinger
    Pages 193-208

  20. Chromatin Assembly in a Crude Fraction From Yeast Cells

    • Karen M. Robinson, Michael C. Schultz
    Pages 209-223
Back to top

Keywords

About this book

In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.

Reviews

From Reviews of the First Edition...

"...will benefit the apprentices...and the experienced scientist venturing into a new technique or changing yeast species...should join the other 'cookery' books on the laboratory shelf." -Journal of Emergency Medicine

"...a useful addition to life sciences libraries and to laboratories embarking on studies with yeast"
-Quarterly Review of Biology

Editors and Affiliations

  • Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    Wei Xiao

Bibliographic Information

  • Book Title: Yeast Protocols

  • Editors: Wei Xiao

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599583

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-437-1Published: 01 September 2005

  • Softcover ISBN: 978-1-61737-569-9Published: 19 November 2010

  • eBook ISBN: 978-1-59259-958-5Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 392

  • Number of Illustrations: 104 b/w illustrations

  • Topics: Mycology, Cell Biology, Biochemistry, general

Publish with us

Policies and ethics

Back to top

Search

Navigation