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Circadian Rhythms

Methods and Protocols

  • Book
  • © 2007

Overview

Editors:
  1. Ezio Rosato

    1. Department of Genetics, University of Leicester, Leicester, UK

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  • Methods for analysis of rhythmic readouts in select model organisms
  • Novel techniques for identifying clock genes by mutagenesis and mircroarrays
  • Procedures for isolation, extraction, and analysis of RNA
  • Procedures for extraction, fractionation, and purification of proteins
  • Detailed in vitro protocols for circadian research
  • Methods for microscopy analysis in circadian biology
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 362)

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Table of contents (44 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Overviews

    1. Front Matter

      Pages 1-1
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    2. Light, Photoreceptors, and Circadian Clocks

      • Russell G. Foster, Mark W. Hankins, Stuart N. Peirson
      Pages 3-28

    3. Statistical Analysis of Biological Rhythm Data

      • Harold B. Dowse
      Pages 29-45

  3. Rhythmic Readouts

    1. Front Matter

      Pages 47-47
      Download chapter PDF

    2. Rhythmic Conidiation in Neurospora crassa

      • Cas Kramer
      Pages 49-65

    3. Monitoring and Analyzing Drosophila Circadian Locomotor Activity

      • Mauro A. Zordan, Clara Benna, Gabriella Mazzotta
      Pages 67-81

    4. Automated Video Image Analysis of Larval Zebrafish Locomotor Rhythms

      • Gregory M. Cahill
      Pages 83-94

    5. Locomotor Activity in Rodents

      • Gianluca Tosini
      Pages 95-101

    6. Analysis of Circadian Leaf Movement Rhythms in Arabidopsis thaliana

      • Kieron D. Edwards, Andrew J. Millar
      Pages 103-113

    7. Detection of Rhythmic Bioluminescence From Luciferase Reporters in Cyanobacteria

      • Shannon R. Mackey, Jayna L. Ditty, Eugenia M. Clerico, Susan S. Golden
      Pages 115-129

    8. Analysis of Rhythmic Gene Expression in Adult Drosophila Using the Firefly Luciferase Reporter Gene

      • Ralf Stanewsky
      Pages 131-142

    9. Monitoring Circadian Rhythms in Arabidopsis thaliana Using Luciferase Reporter Genes

      • Anthony Hall, Paul Brown
      Pages 143-152

  4. Identification of Clock Genes

    1. Front Matter

      Pages 153-153
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    2. Specialized Techniques for Site-Directed Mutagenesis in Cyanobacteria

      • Eugenia M. Clerico, Jayna L. Ditty, Susan S. Golden
      Pages 155-171

    3. Novel Strategies for the Identification of Clock Genes Neurospora With Insertional Mutagenesis

      • Kruno Sveric, Moyra Mason, Till Roenneberg, Martha Merrow
      Pages 173-185

    4. Mutagenesis With Drosophila

      • Patrick Emery
      Pages 187-195

    5. Mutagenesis in Arabidopsis

      • Jodi Maple, Simon G. Møller
      Pages 197-206

    6. Yeast Two-Hybrid Screening

      • Jodi Maple, Simon G. Møller
      Pages 207-223

    7. Microarrays

      • Ken-ichiro Uno, Hiroki R. Ueda
      Pages 225-243
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Keywords

About this book

The aim of Circadian Rhythms is to provide a resource that can be adopted by several types of users: those who are new to circadian biology, those who are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or undertaking comparative studies and would like to consult protocols applied to different organisms before starting the study of new species. This book features a full range of methods that illustrate procedures that have been recently been introduced in circadian studies and by presenting variations to take into account the peculiarities of different model systems.

Editors and Affiliations

  • Department of Genetics, University of Leicester, Leicester, UK

    Ezio Rosato

Bibliographic Information

  • Book Title: Circadian Rhythms

  • Book Subtitle: Methods and Protocols

  • Editors: Ezio Rosato

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-257-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-417-3Published: 02 February 2007

  • Softcover ISBN: 978-1-61737-553-8Published: 19 November 2010

  • eBook ISBN: 978-1-59745-257-1Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 578

  • Number of Illustrations: 115 b/w illustrations

  • Topics: Biochemistry, general, Cell Biology, Animal Genetics and Genomics, Plant Genetics and Genomics

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